丹酚酸B预处理对心肌缺血/再灌注损伤能量代谢的影响

目的 观察丹酚酸B预处理对大鼠心肌缺血/再灌注损伤（MI/RI）能量代谢的作用。方法 通过结扎冠状动脉30min再灌注2 h建立大鼠MI/RI模型，随机分为4组：假手术组、模型组及丹酚酸B高、低（20、10 mg/kg）组，于建立模型前7 d开始ip给药，每天1次；再灌注结束后，采用比色法测定血清乳酸脱氢酶（LDH）、肌酸激酶（CK）活力，染色法测定心肌梗死面积（MIA），定磷法测定心肌组织Na⁺-K⁺-ATP酶、Ca²⁺-Mg²⁺-ATP酶活性。结果 与模型组（42.60%）比较，丹酚酸B高、低剂量组的MIA分别缩小至35.93%和37.21%，差异显著（P<0.05）；与模型组比较，丹酚酸B高、低剂量组血清CK、LDH活力均显著降低（P<0.05、0.01）；与模型组比较，丹酚酸B高、低剂量组心肌组织Na⁺-K⁺-ATP酶、Ca²⁺-Mg²⁺-ATP酶活性均显著升高（P<0.05、0.01）。结论 丹酚酸B预处理可保护MI/RI所致心肌损伤，作用途径可能与改善心肌组织的能量代谢相关。

Salvianolic acid B pretreatment protects myocardial ischemia/reperfusion injury through improvement of energy metabolism

Objective To investigate the effects of Salvianolic acid B (Sal B) pretreatment against myocardial ischemia/reperfusion injury (MI/RI) in rats. Methods MI/RI rats model was established by 30 min of coronary artery ligation and subsequent 2 h of reperfusion. Rats were randomly divided into four groups (n=10):sham group, model group, and Sal B low (10 mg/kg) and high dose (20 mg/kg) group. Before the modeling operation, Sal B was injected intraperitoneal once daily for 7 d. After the modeling operation, the activities of creatine kinase (CK) and lactate dehydrogenase (LDH) in serum of rats were detected by chromatometry, myocardial infarction area (MIA) in rats were examined by staining method and Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase activities in myocardial tissues were determined by phosphorus method. Results Compared with model group, high and low doses of Sal B significantly reduced MIA to 35.93% and 37.21%, respectively (P<0.05). Compared with model group, the activities of serum CK and LDH activities in Sal B high and low dose groups were significantly decreased (P<0.05, 0.01); Compared with model group, the activities of Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase in myocardium of Sal B high and low dose groups were significantly increased (P<0.05 and 0.01). Conclusion Sal B pretreatment could protect MI/RI, and its effects maybe related with the improvement of myocardial energy metabolism.