一测多评法测定不同丹参制剂中4个酚酸成分

目的 建立同时适用于5种丹参制剂（丹参片、复方丹参片、冠心丹参胶囊、心可舒片、双丹胶囊）中4个酚酸类成分一测多评的含量测定方法。方法 采用高效液相色谱法，以丹参素为内参物，建立原儿茶醛、迷迭香酸、丹酚酸B与丹参素的相对校正因子，比较相对保留值法和保留时间差法对成分定位的影响，并采用外标法验证结果的可靠性。结果 采用相对保留值法可对待测成分进行准确定位，所建立的相对校正因子耐用性良好，原儿茶醛、迷迭香酸、丹酚酸B相对于丹参素的相对保留值分别为1.894、5.379、6.599，相对校正因子分别为6.611、3.845、2.059，采用外标法验证结果无显著性差异。结论 该方法稳定、可靠，可为上述丹参制剂质量控制提供参考。

Study on the determination of four phenolic acids in different Danshen preparations by QAMS

Objective A simple, rapid and low-cost quantitative analysis method of multi-components by single marker (QAMS) was developed to the determination of danshensu, protocatechuic aldehyde, rosmarinic acid and salvianolic acid B in Danshen preparations (Danshen Tablet, Fufang Danshen Tablet, Guanxin Danshen Capsule, Xin-Ke-Shu Tablet, Shuangdan Capsule). Methods Danshensu was selected as the internal reference standard, and the relative correction factors were calculated between other three components with danshensu. The relative retention value method and the retention time difference method were compared on the component localization. The external standard method was used to verify the reliability of the QAMS results. Results The tesults showed the relative correction factors of protocatechuic aldehyde, rosmarinic acid and salvianolic acid B were 6.611, 3.845, 2.059, respectively. The relative retention times of protocatechuic aldehyde, rosmarinic acid and salvianolic acid B were 0.314, 0.518, and 0.561, respectively, for accurate location. The QAMS method could assure to obtain consistent results with the external standard method. Conclusion This proposed QAMS method could provide an effective strategy for routine quantitative analysis and quality control of Danshen preparations.