鱼腥草注射液与新鱼腥草素钠注射液体外肝微粒体代谢研究

目的 进行鱼腥草注射液与新鱼腥草素钠注射液体外人肝微粒体代谢研究,为进一步阐明中药多成分体系体内代谢规律奠定基础。方法 制备人肝微粒体,用Brad ford法测定酶活性,配制肝微粒体体外温孵体系,加入鱼腥草注射液与新鱼腥草素钠注射液温孵,用气相色谱-质谱联用（GC/MS）法测定并比较鱼腥草注射液及新鱼腥草素钠注射液代谢样品图谱。结果 检测到鱼腥草注射液的人肝微粒代谢产物20个,新鱼腥草素钠注射液肝微粒代谢产物24个,鱼腥草注射液多成分体系与新鱼腥草素钠注射液单成分经肝微粒体代谢其产物的数目不完全相同,但产物结构类型一致,均为脂肪族、芳香族、萜类挥发性成分,其中两者的代谢产物中均有己醛、α-哌烯、桉树脑、癸酸、庚醇、2-异丙基-5-甲基-1-庚烷6种成分,分别占代谢产物总量的15.40%和49.14%。结论 多成分代谢与单成分代谢规律有所不同,但均呈多成分群聚集的特点,成分之间存在与原植物相似的代谢途径,符合中药各成分在同一网络体系相互连通、交流、传递的“网通性”规律。

Metabolism of Houttuyniae injection and sodium new Houttuyfonate injection in liver microsomes

Objective Studying the metabolism of Houttuyniae and sodium new Houttuyfonate injection in liver microsomes to lay a foundation for clarifying the metabolism rule of Chinese herbal multi components system in vivo and Determining the Q-Marker of houttuynia. Methods The liver microsome was prepared and the enzymatic activity was determined by Brad ford method. The in vivo iver microsomes incubating system was prepared, and the Houttuyniae injection and sodium new Houttuyfonate injection were incubated in the liver microsomes; the metabolic product were determined by GC-MS. Results Houttuynia injections incubated by liver microsomes had 20 metabolites, and 24 metabolites of sodium new Houttuynia injections, of which there were 6 common products. The number of products metabolized by hepatic microsomes in the multi-component system of sodium new Houttuyfonate injection was not exactly the same as that in the single component of Houttuynia cordata injection, but the structure types of products were the same, they were all aliphatic, aromatic and terpene volatile components. The same six metabolites of both components are hexanal, alpha-piperine, eucalyptus, decanoic acid, heptanol alcohol and 2-isopropyl -5-methyl -1-heptane, accounting for 15.40% and 49.14% of the total metabolites respectively. Conclusion The regulation of multicomponent metabolism is different from that of single component, but both of them have the characteristics of multicomponent group aggregation. There is a metabolic pathway similar to the original plant among components. It is in accordance with the law of "network communication" that the components of Chinese medicine are connected, exchanged and transmitted in the same network system.