葱白提取物对非酒精性脂肪肝模型大鼠ATPase6、ATPase8表达的影响

目的]观察葱白提取物对非酒精性脂肪肝(NAFLD)模型大鼠ATP合酶Fo亚基6(ATPase6)、ATP合酶Fo亚基8(ATPase8)表达的影响,并探讨其作用机制。方法]将50只雄性SD大鼠随机分为正常组、模型组、葱白低剂量组、葱白中剂量组、葱白高剂量组,每组10只。采用高脂饮食喂养复制NAFLD模型大鼠,造模10周后,给予葱白提取物连续干预4周后,测定血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)和甘油三酯(TG)、胆固醇(TC)的含量;苏木精-伊红(HE)染色法观察肝脏组织结构变化;Real time PCR检测大鼠肝脏组织中ATPase6、ATPase8 mRNA的表达。结果]与正常组比较,模型组出现明显肝脂肪变性,血清ALT、AST、TC、TG水平明显升高(P0.05),ATPase6、ATPase8 mRNA的表达明显降低(P0.05);相较于模型组,葱白低、中、高剂量组肝脂肪变性均不同程度减轻;血清ALT、AST、TC、TG水平均不同程度降低(P0.05),ATPase6、ATPase8 m RNA表达不同程度升高(P0.05)。与葱白低剂量组比较,葱白中、高剂量组肝脂肪变性减轻明显,ALT、AST、TC、TG水平明显降低(P0.05),ATPase6、ATPase8 mRNA水平明显升高(P0.05),中、高剂量葱白组之间差异无统计学意义。结论]葱白提取物能够改善肝脂肪变性,可能与提高ATPase6、ATPase8的表达,增加肝线粒体ATP合成有关。

Effects of fistular onion stalk extract on the expression of ATPase6 and ATPase8 on non-alcoholic fatly liver disease model rats

Objective] To observing the expression of ATPase6 and ATPase8 and explore the probable mechanism of fistular onion stalk extract in treating nonalcoholic fatty liver disease model in rats.Methods] The 50 male SD rats were randomly divided into normal group, model group, low-dose fistular onion stalk group, medium dose fistular onion stalk group, high-dose fistular onion stalk group, and 10 in each group. The rats were fed with a high-fat diet to replicate the NAFLD model. After 10 weeks of modeling, the extract of the onion was given for 4 weeks. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and triglyceride (TC) and cholesterol (TG) were measured. The changes of liver tissue structure wereexpression of ATPase6 and ATPase8 mRNA in liver tissues of rats.Results] Compared with the normal group, the model group showed obvious hepatic steatosis. The level of serum ALT, AST, TC and TG were higher than normal group(P<0.05). The expression of ATPase6 and ATPase8 mRNA was significantly decreased(P<0.05). Compared with the model group, the lipid degeneration in the liver was alleviated, the level of serum ALT, AST, TC and TG were all decreased, the expression of ATPase6 and ATPase8 mRNA were significantly increased in low-dose fistular onion stalk group, medium dose fistular onion stalk group and high-dose fistular onion stalk group(P<0.05).Conclusion] Fistular onion stalk extract can improve hepatic steatosis, which may be associated with improved expression of ATPase6 and ATPase8, and increased mitochondrial ATP synthesis.