| 续断“发汗”前后水煎液及含药血清对人成骨样MG-63细胞、幼鼠成骨细胞增殖的影响 |
| |
| 引用本文： | 杨莹,杜伟锋,康显杰,来平凡,屠珏. 续断“发汗”前后水煎液及含药血清对人成骨样MG-63细胞、幼鼠成骨细胞增殖的影响[J]. 中草药, 2018, 49(23): 5594-5599 |
| |
| 作者姓名： | 杨莹 杜伟锋 康显杰 来平凡 屠珏 |
| |
| 作者单位： | 浙江中医药大学 药学院, 浙江 杭州 310053;浙江中医药大学附属广兴医院, 浙江 杭州 310007,浙江中医药大学 中药炮制技术研究中心, 浙江 杭州 311401,浙江中医药大学 药学院, 浙江 杭州 310053,浙江中医药大学 药学院, 浙江 杭州 310053,浙江中医药大学动物实验研究中心, 浙江 杭州 310053 |
| |
| 基金项目： | 国家自然科学基金青年科学基金项目（81303224）;浙江中医药大学科研基金项目-2018国家自然科学基金预研项目（2018ZG34）;中华中医药学会青年人才托举工程项目（QNRC2-C12） |
| |
| 摘 要： | 目的探讨续断"发汗"前后水煎液及含药血清对人成骨样MG-63细胞、成骨细胞增殖的影响。方法将人成骨样MG-63细胞、幼鼠成骨细胞分别与续断"发汗"前后水煎液及大鼠含药血清共同培养。MTT法检测细胞增殖,硝基苯磷酸盐法检测成骨细胞的碱性磷酸酶(ALP)活性。结果续断水煎液和含药血清均能显著促进MG-63细胞和成骨细胞增殖(P0.01),并能显著提高成骨细胞的ALP活性(P0.01),且相同剂量未"发汗"组作用普遍优于或非劣于"发汗"组。结论结合前期成分研究,推断续断"发汗"前后成分的改变影响了其促进细胞增殖和分化的作用,为其进一步研究奠定基础。
|
| 关 键 词： | 续断 发汗 MG-63细胞 成骨细胞 细胞增殖 碱性磷酸酶 |
| 收稿时间： | 2018-03-08 |
Effect of decoction and drug-containing serum of sweated and crude Radix Dipsaci on proliferation of MG-63 cells and osteoblasts |
| |
| YANG Ying,DU Wei-feng,KANG Xian-jie,LAI Ping-fan and TU Jue. Effect of decoction and drug-containing serum of sweated and crude Radix Dipsaci on proliferation of MG-63 cells and osteoblasts[J]. Chinese Traditional and Herbal Drugs, 2018, 49(23): 5594-5599 |
| |
| Authors: | YANG Ying DU Wei-feng KANG Xian-jie LAI Ping-fan TU Jue |
| |
| Affiliation: | College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, China;Guang Xing Hospital Affiliated to Zhejiang University of Traditional Chinese Medicine, Hangzhou 310007, China,Research Center of Traditional Chinese Medicine Processing Technology, Zhejiang Chinese Medical University, Hangzhou 311401, China,College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, China,College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, China and Animal Experimental Research Center, Zhejiang Chinese Medical University, Hangzhou 310053, China |
| |
| Abstract: | Objective To investigate the effect of decoction and drug serum of sweated and crude Dipsaci Radix on the proliferation of human osteoblast-like cells (MG-63) and osteoblasts. Methods MG-63 cells and osteoblasts were co-cultured with decoction and rat serum containing Dipsaci Radix before and after "sweating". The cell proliferation was detected by MTT method and the ALP activity of osteoblasts was detected by nitrophenyl phosphate method. Results Both decoction and drug-containing serum can significantly promote the proliferation of MG-63 cells and osteoblasts (P < 0.01), and significantly increase the ALP activity of osteoblasts (P < 0.01). Moreover, sweated group were generally better than or non-inferior to crude group at the same dose concentration of two administration methods. Conclusion Combined with component studies, it can be inferred that the changes in composition before and after "sweating" affect its role in promoting cell proliferation and differentiation, with view to laying the foundation for further research. |
| |
| Keywords: | Dipsaci Radix sweating human osteoblast-like MG-63 cells osteoblasts cell proliferation ALP |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中草药》浏览原始摘要信息 |
|
点击此处可从《中草药》下载全文 |
|
