ATRA attenuate proteinuria via downregulation of TRPC6 in glomerulosclerosis rats induced by adriamycin |
| |
| Authors: | Lei Zhang Xiu-Ping Chen He Qin Ling Jiang |
| |
| Affiliation: | 1. Department of Pediatric Nephrology, The First Affiliated Hospital of GuangXi Medical University, Nanning, China;2. Department of Pediatric, Affiliated Hospital of Hebei University, Baoding, China |
| |
| Abstract: | Objective: In this research, we explored the molecular mechanism of proteinuria in glomerulosclerosis rats and the protective effects of ATRA.Methods: This research set up three groups: SHO group, GS group, and ATRA group (15?mg/(kg d), Sigma, St. Louis, MO). The serum creatinine (Scr), urea nitrogen (BUN), and 24-h proteinuria were detected 12?weeks after administration of ATRA. The pathological and ultrastructure changes were observed under light microscope and transmission electron microscope. The protein expression of TGF-β1 and Col-IV in glomerulus was detected by immunohitochemistry method. The mRNA and the protein expression of glomerular TRPC6 were detected by RT-PCR and Western blot.Results: In the rat model of GS, the expressions of TRPC6 were significantly elevated compared with the normal rat group; however, the use of ATRA down-regulated the expression of TRPC6 in the glomeruli and attenuated glomerulosclerosis and proteinuria. Scr and BUN were also improved by the treatment of ATRA.Conclusions: Our results demonstrated that ATRA could ameliorate glomerulosclerosis and proteinuria in GS, which may be related to suppressed expression of TRPC6. |
| |
| Keywords: | ATRA glomerulosclerosis proteinuria TRPC6 |
|
|
