Ｔｉｍ-３在胃癌组织中的表达及其临床意义

目的　研究Ｔ细胞免疫球蛋白及黏蛋白结构域分子３（Ｔｉｍ-３）在胃癌组织中的表达及其临床意义。方法　采用流式细胞术检测５２例胃癌及１５例胃炎患者手术标本Ｔｉｍ ３的表达。结果　ＣＤ４＋和ＣＤ８＋Ｔ细胞均可见Ｔｉｍ-３的表达，胃癌组织ＣＤ４＋Ｔ细胞Ｔｉｍ-３表达为（１２.４±８.４１）％，明显高于胃炎组的（６.０１±１.９５）％；胃癌组织ＣＤ８＋Ｔ细胞中Ｔｉｍ-３表达为（９.５６±７.４８）％，明显高于胃炎组的（５.３９±２.９８）％，差异均有统计学意义（Ｐ＜００５）。Ｔｉｍ-３在ＣＤ４＋Ｔ细胞中的表达与胃癌患者的浸润深度、淋巴结转移和ＴＮＭ分期有关（Ｐ＜０.０５），而与性别、年龄、肿瘤大小、组织学类型均无关（Ｐ＞０.０５）。Ｔｉｍ-３在ＣＤ８＋Ｔ细胞中的表达仅与胃癌患者的ＴＮＭ分期有关（Ｐ＜０.０５），而与其他临床病理学特征均无关（Ｐ＞０.０５）。结论　Ｔｉｍ-３在胃癌的侵袭、转移过程中可能起着重要作用，是胃癌患者的不良预后因素之一。

Expression and clinical significance of Tim-3 in gastric cancer tissues

Objective To evaluate the expression of Tim-3 and its clinical significance in the gastric cancer tissues.Methods Fifty-two gastric cancer specimens and 15 gastritis tissues were detected for Tim-3 by flow cytometry,and 15 specimens of gastritis tissues served as the control.Results Tim-3 expressed both in gastric cancer tissues and gastritis tissues.In CD4＋T cells,the expression level of Tim-3 in gastric cancer tissues（12.4±8.41）% was significantly higher than gastritis tissues（6.01±1.95）%;In CD8＋T cells,the expression level of Tim-3 in gastric cancer tissues（9.56±7.48）% was significantly higher than gastritis tissues（5.39±2.98）%（P0.05）.Tim-3 expression in CD4＋T cells was significantly associated with clinicopathological features such as invasion depth,lymph node metastasis and TNM stage（P0.05）.No correlation was found between Tim-3 and gender,age,tumor size and histological type（P0.05）.In CD8＋T cells,Tim-3 expression correlated with TNM stage rather than other clinicopathological features.Conclusion Tim-3 played an important role in the invasion and metastasis of gastric cancer,and was a prognostic factor for poor outcome.