环磷酰胺或塞替派诱导人支气管上皮细胞恶性转化的形态计量学

以永生化人支气管上皮细胞 (BEAS- 2 B)分别受环磷酰胺 (BEAS- CP) ,塞替派 (BEAS- TE)暴露并发生转化的细胞为模型 ,对转化细胞进行形态计量学研究 ,期望寻找人类上皮细胞恶性转化的形态计量指标 .结果表明 :BEAS- 2 B经两种药物处理后可生成转化型和非转化型细胞集落 .转化细胞的形态计量参数中 ,细胞和细胞核面积 ,最大直径 ,最小直径 ,等效直径 ,形状因子的数值按 BEAS- 2 B,BEAS- CP,BEAS- TE的组序递升 .表达细胞形态为异形的指标 :细胞和细胞核异形指数 ,核浆比则沿BEAS- 2 B,BEAS- CP,BEAS- TE的顺序递减 .结果提示 ,上述改变可能是细胞由正常趋向恶性增殖的形态特征

Cytological morphometry of human bronchial epithelial cells transformed by cyclophosphamide or thiotepa

Cytological morphometry of im- mortalized human bronchial epithelial cell line(BEAS- 2 B) transformed by cyclophos- phamide(BEAS- CP) or thiotepa (BEAS- TE) was studied. Parameters of trans- formed cells including cellular and nuclear area,maximal diameter,minimal diameter, diameter equivalent and form factor were significantly increased in the order of BEAS- 2 B,BEAS- CP and BEAS- TE cell line.Parameters including irregular form index,nucleus- cytoplasm ratio,which de- picted the irregular shape of cell were de- creased as the same order.The result was consistently paralleled with the change of cell kinds which consisted of transformed and nontransformed cell line.In the cell transformation process the number of ciliat- ed cells became lesser and proliferating basal cells increased.The data suggest that parameters of cytological morphometry are important in investigation of the transfor- mation of human bronchial epithelial cells.