| HPLC法测定骨伤愈合胶囊中三七皂苷R1的含量 |
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| 引用本文： | 唐何,;毛谊平,;林海,;李子君,;任荣军.HPLC法测定骨伤愈合胶囊中三七皂苷R1的含量[J].中国医药指南,2014(25):6-6. |
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| 作者姓名： | 唐何 ;毛谊平 ;林海 ;李子君 ;任荣军 |
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| 作者单位： | [1]湖南省食品药品检验研究院,湖南长沙410000; [2]岳阳市食品药品检验所,湖南岳阳414000; [3]湖南省中药不良成分快速检测及脱除工程技术研究中心,湖南长沙410208 |
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| 基金项目： | 2013年湖南省科技计划项目（编号：2013SK5072） |
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| 摘 要： | 目的建立HPLC法测定骨伤愈合胶囊中三七皂苷R1含量。方法色谱柱为Agilent ZORBAX SB-C18(250 mm×4.6 mm,5μm);流动相为乙腈-0.4%磷酸溶液梯度洗脱,流速为1.0 mL/min,检测波长为203 nm,柱温为30℃。结果三七皂苷R1在0.20482.0480μg范围内线性关系良好,r=0.9999(n=5),平均回收率为99.0%(n=9),RSD为0.8%。结论该方法灵敏、准确、专属性强,能简便有效控制骨伤愈合胶囊中三七皂苷R1的含量。
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| 关 键 词： | 高效液相色谱 骨伤愈合胶囊 三七皂苷R1 |
Determination of Notoginsenoside R1 in Gushang Yuhe Capsules by HPLC |
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| Institution: | TANG He, MAO Yi-ping, LIN Hai, LI Zi-jun, REN Rong-jun ( 1 Hunan Institute Food and Drug Control, Changsha 414000, China; 2 Yueyang Institute Food and Drug Control, Yueyang 414000, China; 3 Hunan Research Center of Engineering Technology for Rapid Test and Removal of Toxic and Harmful Substances in Chinese Medicine, Changsha 410208, China) |
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| Abstract: | Objective To establish an HPLC method for determination the content of Notoginsenoside R1 in Gushang Yuhe Capsules. Methods The column was Agilent ZORBAX SB-C18（250 mm×4.6 mm,5 μm）and the temperature was 30 ℃;The mobile phase was acetonitrile-0.4% phosphoric acid solution with linear gradient elution, detection was done at 203 nm and a flow rate of 1.0 mL/min. Results Notoginsenoside R1 has a good linear relationships in 0.2048～2.048 μg（r=0.9999）;the average recovery was 98.97%（n=9,RSD was 0.8%. Conclusion The methods are sensitive, accurate and specific,which can be used for the quality control of Gushang Yuhe Capsules. |
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| Keywords: | HPLC Gushang Yuhe Capsules Notoginsenoside R1 |
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