黄芪注射液对体外血管新生的影响*

背景：血管新生受生长因子的影响，黄芪可与血管内皮生长因子具有协同促血管新生的功效，但机制尚不明确。目的：通过与单一血管内皮生长因子干预比较，观察中药黄芪对体外血管新生的作用机制。方法：将黄芪注射液及血管内皮生长因子作用于SD大鼠胸主动脉内皮细胞，应用细胞增殖、细胞迁移、小管形成实验观察黄芪注射液对体外血管新生的促进作用，用Western blot实验检测血管内皮生长因子的表达。结果与结论：黄芪能明显促进大鼠胸主动脉内皮细胞的增殖(P < 0.01)，迁移的细胞数增加(P < 0.01)，胸主动脉内皮细胞的小管形成数增加(P < 0.01)，并明显促进内皮细胞血管内皮生长因子的表达(P < 0.01)。说明黄芪能明显促进内皮细胞增殖、细胞迁移、小管形成，具有显著的促进体外血管新生作用，其机制可能是通过增加血管内皮生长因子的表达而实现的。

Effect of Radix Astragali injection on angiogenesis in vitro

BACKGROUND: Angiogenesis affects by growth factor, Radix Astragali and vascular endothelial growth factor (VEGF) have synergistic effect on angiogenesis, but its mechanism remains unclear.OBJECTIVE: To observe the effects of Radix Astragali injection on the mechanism of in vitro angiogenesis by comparing with intervention in a single VEGF.METHODS: Radix Astragali injection and VEGF was cultured with aortic endothelial cells. The promotion of Radix Astragali injection on in vitro angiogenesis was observed by tests of cell proliferation, cell migration and tube formation. The VEGF expression was determined by Western blot.RESULTS AND CONCLUSION: Radix Astragali injection could promote rat aortic endothelial cell proliferation (P < 0.01), increase the number of migrated cells (P < 0.01), induce aortic endothelial cells increase the number of tube formation, and significantly increase VEGF expression (P < 0.01). The findings demonstrated that, Radix Astragali injection can promote endothelial cell proliferation, migration and tube formation and has a significant effect on promoting in vitro angiogenesis, which achieved by increasing VEGF expression.