应用活体内光学成像技术观察裸鼠乳腺癌模型的建立

目的 应用活体内光学成像技术观察裸鼠乳腺癌模型的建立.方法 将携带荧光素酶(luc)基因的乳腺癌细胞MDA-MB-231-lue注入裸鼠体内,采用"活体内光学成像系统"观察乳腺癌模型的建立.结果 裸鼠乳腺脂肪垫种植MDA-IVlB-23-ldue后原位成瘤,肿瘤的体积和光子数在第2周到第5周时都呈上升趋势;MDA-IVIB-231-lue细胞尾静脉注射后肿瘤病灶主要位于肺部,左心室注射后可形成全身多发肿瘤病灶.结论 活体内光学成像系统能够很好地显示裸鼠乳腺癌原位成瘤和尾静脉注射、左心室注射模拟转移瘤模型体内成瘤的过程.

The establishment of an athymic mouse model bearing breast cancer monitored by an in vivo optical imaging system

Objective To establish the athrmic inouse model of breast cancer in normal position and imitated metastatic breast cancer. Methods Breast cancer cells MDA-MB-231-luc carrying luciferase gene was injected into the athymie mice.The optieal imaging in vivo system was used to observe the establislament of the model. Reseults The breast tumor emerged after we planted the MDA-MB-231-lue cells in the mammary gland fatpads,the volume and photon of the tumor increased during the second weekto the fifth week.After injection by the tail vein,the tumors mainly located in the lungs While after infusion in the left alrtrium.the tumolrs metastate to all over the body. Conclusions We succeeded in the establishment of the athymic mice model of breast cancer.in situ and imitated metastatic breast cancer by iniection into the vena caudalis and the left alttrium.The optical imaging in vivo system could distinctly show the formation of the tumors.