盐酸戊乙奎醚不同给药途径对大鼠急性肺损伤作用比较

目的观察雾化吸入和静脉应用盐酸戊乙奎醚对油酸诱导大鼠急性肺损伤（acute lung injury，ALI）的影响。方法采用静脉注射油酸复制ALI大鼠模型。将30只SD大鼠随机分为3组：ALI组、盐酸戊乙奎醚雾化吸入治疗组和盐酸戊乙奎醚静脉注射治疗组。实验过程中每隔60min记录大鼠血压（BP）、肺动脉压（PAP）并且检验动脉以及混合静脉血气。注射油酸240分钟后检测支气管肺泡灌洗液（BALF）蛋白定量、肺髓过氧化物酶（myeloperoxidase，MPO）活性以及血清肿瘤坏死因子-α（Tumor necrosis factor—alpha，TNF-α）浓度。结果雾化吸入组较之静脉注射组更能缓解由于注射油酸引起的PAP升高、Paw升高和PaO2／FiO2下降。静脉注射组血压下降明显，与其它二组相比在180min以后出现显著性差异。雾化吸入组和静脉注射组BALF蛋白定量、白细胞计数，肺MPO，血清TNF-α均比ALI组降低，雾化吸入组降低更为明显。结论盐酸戊乙奎醚能够缓解油酸引起的ALI多项指标，相同剂量条件下雾化吸入较之静脉注射途径对ALI有更好的治疗作用。

Effect of penehyclidine hydrochloride on OA induced rat acute lung injury:a comparison between different administration methods

Aim To investigate the therapeutic effect of penehyclidine hydroehloride in rats with oleic acid（OA）induced acute lung injury （ALI） ,and compare the effects between inhalation and intravenous therapy. Methods 30 male Sprague-Dawley rats were randomly divided into 3 equal groups：ALI group,penehyclidine hydrochloride inhalation group,and intravenous penehyclidine hydroehloride group. Blood pressure（BP） ,pulmonary artery pressure（PAP） ,artery blood gas analysis and mixed venous blood gas analysis were determined every 60 minutes during the experiment. The neutrophils and total protein（TP） content in bronehoalveolar lavage fluid （BALF） ,lung myeloperoxidase（MPO） activity, and the serum tumor necrosis factor - alpha （TNF-ct） concentration were determined after the experiments in each group. Results The blood pressure was lower in intravenous group than that in other 2 groups. PAP, Paw and PaO2/FiO2 were improved in inhalation group and intravenous group,eornpared with ALI group. TP and neutrophils account in BALF,lung MPO activity and serum TNF-α concentration was lower in inhalation group and intravenous group than those in ALI group. Most parameters above are even better in inhalation group. Conclusion The improvement in the ALI model brought by inhaled penehyclidine hydroehloride is more effective than intravenous way.