Sperm DNA fragmentation in the total and vital fractions before and after density gradient centrifugation: Significance in male fertility diagnosis |
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| Affiliation: | 1. Unite de Diabetologie et Nutrition, Université Catholique de Louvain (Cliniques Universitaires St-Luc);2. University of Utah School of Medicine;3. Dept of Orthopedic Surgery, Cliniques Universitaires Saint-Luc;1. Academic Department of Medical Genetics, University of Cambridge, Cambridge, United Kingdom;2. Centre for Liver Research and NIHR Biomedical Research Unit, University of Birmingham, Birmingham, United Kingdom;1. CIMAB, Barcelona Male Infertility Centre, Sant Quirze del Vallés, Barcelona, Spain;2. Departament de Biologia Cel·lular, Fisiologia i Immunologia, Facultat de Medicina, Universitat Autònoma de Barcelona (UAB), Bellaterra, Barcelona, Spain;3. Clínica GINEFIV, General Life IVF, Madrid, Spain |
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| Abstract: | BackgroundSperm DNA fragmentation measured by different techniques make comparisons impossible due to lack of standardization. Induction of DNA damage after sperm preparation in the entire fraction has been observed on independent occasions but findings are not consistent.MethodsMen presenting at a University hospital setup for infertility treatment. DNA damage via TUNEL assay was validated on fresh semen samples, as conventional semen parameters, to reduce variability of results.ResultsSperm motility in neat semen inversely correlated with sperm DNA fragmentation in the total fraction, but, total count, leukocytes and immature germ cells significantly affected the vital fraction. Sperm DNA fragmentation was observed both in normal and subnormal semen samples, but was significantly different in the total fraction of astheno-, asthenoterato- and oligoteratozoospermic men. After density gradient centrifugation, sperm DNA fragmentation increased significantly in the total but decreased in the vital fraction. Advancing male age significantly influenced damage in the total but not in the vital population.ConclusionsThese findings provide opportunities to investigate the significance of the total and the vital fractions both in natural conception and after different assisted reproductive technologies. |
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