Bacteriospermia,extended spectrum beta lactamase producing Gram-negative bacteria and other factors associated with male infertility in Mwanza,Tanzania: a need of diagnostic bacteriology for management of male infertility |
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Authors: | Vitus Silago Yusuph Mukama Anna L Haule Frank Chacha John Igenge Martha F Mushi Stephen E Mshana |
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Institution: | 1. Department of Microbiology and Immunology, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, Bugando. P. O. Box 1464 Mwanza, Tanzania;2. Department of Urology, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, Bugando. P. O. Box 1464 Mwanza, Tanzania |
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Abstract: | BackgroundInfections caused by Extended spectrum beta lactamase (ESBL) producing bacterial are global challenge. There is limited information on the magnitude of bacteriospermia, ESBL producing Gram-negative bacteria (GNB) causing bacteriospermia and factors associated with male infertility. This study determined magnitude of bacteriospermia, ESBL-GNB and other factors association with infertility among presumptive infertile men in Mwanza, Tanzania.MethodsA cross-sectional hospital-based study was conducted between May 2017 and July 2018 among 137 presumptive infertile men. Semen specimens were self-collected by masturbation into clean, sterile and none-spermicidal containers and processed following laboratory standard operating procedures (SOPs). Data analysis was done using STATA 13.0.ResultsGram-negative bacteria were predominantly isolated (86.4%), of which 31.6% were ESBL producers. In a total 44 bacteria were isolated from semen culture. The blaCTX-M gene was detected in 75% of phenotypically confirmed ESBL producers. Infertility was independently found to be associated with abnormal spermatozoa morphology (OR (95%CI): 14.48(3.17–66.05)) and abnormal spermatozoa motility (OR (95%CI): 0.05(0.01–0.24)). However, neither bacteriospermia (OR (95%CI): 0.86(0.29–2.59)) nor ESBL bacteriospermia (OR (95%CI): 0.13(0.01–1.22)) was found to be associated with infertility.ConclusionOne third of bacteriospermia is due to ESBL-producers with history of antibiotic use being protective factor for infertility. Abnormal spermatozoa morphology and poor spermatozoa forward motility independently predicted infertility. |
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Keywords: | bacteriospermia blaCTX-M male infertility extended spectrum beta lactamase Mwanza Tanzania |
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