Quantitative analysis of biofilm bacteria according to different stages of early childhood caries |
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| Institution: | 1. Faculty of Dentistry, Federal University of Ceará, Sobral, Ceará, Brazil;2. Department of Oral Diagnosis, Piracicaba Dental School, State University of Campinas, Piracicaba, São Paulo, Brazil;3. Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, Ceará, Brazil;1. Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology, Peking University, Beijing, China;2. Central Laboratory, Peking University School and Hospital of Stomatology, Peking University, Beijing, China;1. Ministry of Health, Saudi Arabia;2. Department of Pediatric Dentistry, Riyadh Elm University, Riyadh, Saudi Arabia;3. Department of Microbiology and Immunology, Riyadh Elm University, Riyadh, Saudi Arabia;1. UNESP - Univ. Estadual Paulista, Araçatuba Dental School, Department of Pediatric Dentistry and Public Health, Rua José Bonifácio, 1193, 16015050, Araçatuba, SP, Brazil;2. UNESP - Univ. Estadual Paulista, Araçatuba Dental School, Department of Restorative Dentistry, Rua José Bonifácio, 1193, 16015050, Araçatuba, SP, Brazil;3. UFRGS - Federal University of Rio Grande do Sul, Faculty of Dentistry, Department of Preventive Dentistry, Rua Ramiro Barcelos, 2492, Bairro Santa Cecília, 90035-003, Porto Alegre, RS, Brazil;4. UFRGS - Federal University of Rio Grande do Sul, Faculty of Dentistry, Department of Conservative Dentistry, Rua Ramiro Barcelos, 2492, Bairro Santa Cecília, 90035-003, Porto Alegre, RS, Brazil;1. College of Applied Medical Sciences, Yanbu el Bahr, Taibah University, 5000, Saudi Arabia;2. College of Sciences, Yanbu el Bahr, Taibah University, Saudi Arabia;3. Laboratory of Analyze, Treatment and Valorization of Environmental Polluants and Products, Faculty of Pharmacy, Monastir University, Tunisia;4. Laboratory of Molecular Biology, Ibn el Jazar Hospital, Kairouan, Monastir University, Tunisia;5. Department of Medicine and Oral Surgery, University Hospital, Clinic of Odontology, Monastir University, Tunisia;1. Department of Stomatology, The Children’s Hospital, Zhejiang University School of Medicine, National Clinic Research Center for Child Health, No. 3333 Binsheng Road, Binjiang District, Zhejiang Province, Hangzhou, 310052, PR China;2. Analysis Center of Agrobiology and Environmental Sciences, Zhejiang University, Hangzhou, 310058, No. 866 Yuhangtang Road, Xihu District, Zhejiang Province, PR China;3. Formerly visiting scientist at Zhejiang University, Hangzhou, Zhejiang Province, PR China;4. Amity Institute of Microbial Technology (AIMT), Amity University Uttar Pradesh (AUUP), Sector 125, Super Express Way, Noida, 201 303, UP, India;1. Laboratory of Microbiology and Molecular Biology, Sao Francisco University Dental School, Bragança Paulista, SP, Brazil;2. Department of Pediatric Dentistry, Piracicaba Dental School, University of Campinas, Brazil;3. Department of Operative Dentistry, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, CE, Brazil;4. Federal University of Rio de Janeiro State, Rio de Janeiro, RJ, Brazil |
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| Abstract: | ObjectiveMonitoring selected key species related to health or disease may facilitate caries risk assessment and discovery of novel ecological preventive and therapeutic approaches. This study aimed at quantifying Actinomyces naeslundii, Bifidobacterium spp., Lactobacillus acidophilus, Lactobacillus casei group, Streptococcus gordonii, Mitis group and Streptococcus mutans by quantitative polymerase chain reaction (qPCR) in dental biofilm from Brazilian children with different stages of early childhood caries (ECC).DesignSeventy-five preschool children were clinically evaluated by ICDAS criteria and divided into groups: caries-free (CF; n = 20), enamel caries lesions (ECL; n = 17) and dentine caries lesions (DCL; n = 38). Plaque samples from all children were collected for detection and quantification of the selected bacteria.ResultsL. acidophilus and L. casei group were absent in almost all plaque samples. No differences in relative proportions of A. naeslundii, Mitis group and S. gordonii were observed in any stage of caries. However, S. mutans and Bifidobacterium spp. were present at higher concentrations in the biofilm of children with DCL (p < 0.001). Multivariate analysis showed that S. mutans and Bifidobacterium spp. were strongly associated with biofilm in children with DCL.ConclusionDifferences were observed in the proportion of acidogenic and aciduric bacteria with dental caries progression. The data indicate that S. mutans and Bifidobacterium spp. in dental biofilm may be involved in some progression processes for ECC. |
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| Keywords: | Bacteria Dental plaque Early childhood caries Preschoolers Quantitative polymerase chain reaction |
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