Evidence for programmed odontoblast process retraction after dentine exposure in the rat incisor |
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| Affiliation: | 1. Centre for Oral Health Research, UK;2. Institute of Cellular Medicine, UK;3. School of Dental Sciences Newcastle University, UK;4. University of Baghdad College of Dentistry, Iraq;5. Urology and Urological Rehabilitation Antwerp University, Belgium;1. Centro de Ciências da Saúde, Centro Universitário Serra dos Órgãos, Avenida Alberto Torres, 111, Alto, Teresópolis, Rio de Janeiro 25964004, RJ, Brazil;2. Laboratório de Ciência Radiológicas, Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier, 524, Maracanã, Rio de Janeiro 20550900, RJ, Brazil;3. Setor de Facomatoses do Serviço de Genética Clínica do IPPMG, Universidade Federal do Rio de Janeiro, Avenida Brigadeiro Trompowsky, Rio de Janeiro 21949590, RJ, Brazil;4. Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/n—Campus Universitário, São Pedro, Juiz de Fora 36036900, MG, Brazil;5. Departamento de Ciências Fisiológicas, Instituto Biomédico, Universidade Federal do Estado do Rio de Janeiro, Rua Frei Caneca, 94, Rio de Janeiro 20211040, RJ, Brazil;1. State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Cariology and Endodontics West China Hospital of Stomatology, Sichuan University, China;2. Hospital of Stomatology Wuhan University, Wuhan, China;1. Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido, Japan;2. Division of Biochemistry, Department of Oral Biology, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido, Japan;3. Department of Pathology, Brigham and Women''s Hospital, Boston, Massachusetts 02115;4. Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, New York 10065;5. Department of Microbiology, Brain Korea 21 PLUS Project for Medical Sciences and Department of Orthopaedic Surgery, Yonsei University College of Medicine, Seoul, Republic of Korea;6. Cutaneous Biology Research Center, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts 02129;12. Forsyth Institute, Cambridge, Massachusetts 02142;8. Howard Hughes Medical Institute and Program in Molecular Medicine, Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605;9. Department of Surgery, Massachusetts General Hospital, Shriners Burns Hospital, Harvard Medical School, Boston, Massachusetts 02115;10. Department of Medicine, Weill Cornell Medical College, New York, New York 10065 |
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| Abstract: | ObjectiveTo re-examine the morphology and potential functions of odontoblasts in intact rat incisors and after cavity preparation into dentine.DesignIntact incisors were fixed, decalcified, snap frozen and sectioned (10 μm), before staining with rhodamine phalloidin or antibodies for cyto-skeletal proteins: vimentin and actin, ion transporter: NaK-ATPase, and dendritic cell marker: OX6. Samples with cavity were processed similarly and stained for actin and vimentin before comparing the lengths of odontoblast processes (OP) at baseline, 3 h and 24 h (n = 5 for each group).ResultsActin was expressed through the full length of OP, while vimentin immunoreactivity was not uniform, with 4 distinct regions. OP showed morphological complexity with fine branches emanating within different regions of dentine. Novel actin-positive tree-like OP were identified within predentine which reduced in intensity and length toward the incisal portion of the tooth. Specimens with cavities showed time-dependant pulpal retraction of OP.ConclusionsDifferences in structural antibody expression suggest functional variations in OP within different regions of dentine. The role of actin positive OP in predentine is not known, but could be related to dentine deposition, cellular stability or sensing mechanisms. Cavity preparation into dentine was followed by programmed retraction of OP which could be controlled either mechanically by the spatial limitation of the OP within dentinal tubules or structurally by the presence of vimentin, in addition to actin, in the mid-dentine. |
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| Keywords: | Odontoblast process Actin Rat incisor Pulp physiology Dentine |
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