Inhibitory effects of tea catechin epigallocatechin-3-gallate against biofilms formed from Streptococcus mutans and a probiotic lactobacillus strain |
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| Institution: | 1. Institute of Oral Biology, National Yang-Ming University, Taipei, Taiwan;2. Department of Stomatology, Taipei Veterans General Hospital, Taipei, Taiwan;3. School of Dentistry, National Yang-Ming University, Taipei, Taiwan;4. Department of Community Dentistry, Zhong-Xiao Branch, Taipei City Hospital, Taipei, Taiwan;5. Faculty of Medicine, National Yang-Ming University, Taipei, Taiwan;1. Universidade Federal do Rio de Janeiro (UFRJ), School of Dentistry, Rua Professor Rodolpho Paulo Rocco, 325, Cidade Universitária, Rio de Janeiro 21941913, Brazil;2. Universidade Federal Fluminense (UFF), Faculdade de Medicina Veterinária, Rua Vital Brazil Filho, 64, Niterói, 24230340 Rio de Janeiro, Brazil;3. Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro (IFRJ), Departamento de Alimentos, Rua Senador Furtado, 171, Maracanã, 20270–021 Rio de Janeiro, Brazil;1. The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory for Oral Biomedical Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China;2. VIP Center, Hangzhou Dental Hospital, Hangzhou, China |
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| Abstract: | ObjectiveEffects of tea catechin epigallocatechin-3-gallate (EGCG) against biofilm formation by Streptococcus mutans and probiotic Lactobacillus casei in Yakult® (LcY) were examined.DesignBiofilms were formed by S. mutans alone (Sm) and co-culture of S. mutans and LcY (Sm + LcY) in the absence or presence of EGCG. The biomass of biofilms, which were sonicated or not, was measured by the crystal violet assay. Biofilm morphology was observed by scanning electron microscopy. Bacterial viability and extracellular polysaccharides were determined by SYTO9/propidium iodide and dextran-conjugated fluorescein staining, respectively, and confocal microscopy. Gene expression of glucosyltransferase was determined by quantitative polymerase chain reaction.ResultsWhile 250 μg/ml EGCG significantly decreased the biomass and acid production of Sm biofilms, 500 μg/ml EGCG was required to inhibit Sm + LcY biofilm formation and acid production. EGCG decreased the amount of live bacteria present in both Sm and Sm + LcY biofilms. The level of dead bacteria in Sm + LcY biofilms was higher than in Sm biofilms when formed in the presence of 250 μg/ml EGCG. EGCG decreased levels of extracellular polysaccharides in Sm and Sm + LcY biofilms. The extent of biofilm removal by sonication was not different between Sm and Sm+LcY biofilms formed in the absence or presence of 62.5 or 125 μg/ml EGCG. The level of Sm gtfB and gtfD expression in Sm + LcY biofilms was higher than those in the Sm biofilms when formed in the presence of EGCG at 250 μg/ml.ConclusionThe results indicated that LcY might interfere the inhibitory effects of EGCG against biofilm formation by S. mutans. |
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| Keywords: | Epigallocatechin-3-gallate Probiotic Biofilms |
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