Imaging of Toxocara canis larvae labelled by CFSE in BALB/c mice |
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Authors: | Kolbeková Petra Kolá?ová Libu?e Větvi?ka David Syr??ek Martin |
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Institution: | (1) National Reference Laboratory for Tissue Helminthoses, General University Hospital in Prague, Prague, Czech Republic;(2) Department of Microbiology of the Third Faculty of Medicine, Charles University in Prague, Prague, Czech Republic;(3) Institute of Immunology and Microbiology, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Studničkova 7, 128 00 Prague 2, Czech Republic;(4) Department of Immunology and Gnotobiology, Institute of Microbiology, Academy of Science, Prague, Czech Republic;(5) Department of Pathology, Na Homolce Hospital, Prague, Czech Republic; |
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Abstract: | Mice are used most often as a model for human toxocarosis caused by Toxocara canis larvae. Variety of symptoms developing during the infection reflects behaviour of the larvae, which are able to escape from
the intestine and further invade and damage various host organs. In order to find an approach enabling observation on parasite
behaviour in mouse in vivo, we used an epifluorescence method and a small animal imaging system (SAIS). Larvae of T. canis were labelled by carboxyfluorescein succinimidyl ester (CFSE) which incorporated on the parasite gastrointestinal tract.
Following infection of BALB/c mice by CFSE-labelled larvae it has been observed that staining had no influence on viability
and further migratory activity of the parasites through the host organs (the intestine, liver, lungs and brain) where they
were detected by SAIS until day 17 p.i. In addition, the dye did not affect larval antigenic activity as well as the development
of related immune response. Imaging of parasites labelled by CFSE, therefore, may represent a promising way to study behaviour
of T. canis larvae in a paratenic host. |
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