雷公藤红素对斑马鱼胚胎心脏毒性的初步研究

目的研究雷公藤红素对斑马鱼胚胎的心脏毒性。方法以发育48 h的斑马鱼胚胎为心脏毒性模型,以不同浓度的雷公藤红素处理上述胚胎,分别于处理后6、12、24 h观察胚胎心脏形态和功能变化。结果1μmol.L-1雷公藤红素作用24 h未引起胚胎心脏中毒。而2、3、4μmol.L-1雷公藤红素均导致胚胎心脏中毒,出现心脏线性化、心膜出血、血细胞在心区堆积等现象,而且心率也随着浓度的升高和作用时间的延长而明显下降,引起心率下降的EC50(24 h)约为1.78μmol.L-1。结论雷公藤红素对斑马鱼胚胎具有心脏毒性作用。

Preliminary study on cardiotoxicity of celastrol to zebrafish embryo

Aim To study the cardiotoxicity of celastrol to zebrafish embryo.Method 48 h post-fertilization zebrafish embryos were used as model for analysis of heart toxicity and were treated with various concentrations of celastrol.6,12,24 h after treatment morphological and functional changes of embryos hearts were observed.Results Cardiotoxicity was not found in embryos during 24 h treatment with 1 μmol·L-1 concentration of celastrol.Toxic symptoms of embryos were caused by 2,3,4 μmol·L-1 concentrations of celastrol with appearance of heart linearization,heart membrane hemorrhage and hemocytes accumulation in cardiac region.Moreover,heart rate decreased significantly with increase of concentration and prolongation of treatment.The EC50(24 h)of decrease of heart rate was about 1.78 μmol·L-1.Conclusion Celastrol is cardiotoxic to zebrafish embryo.