乌司他丁对兔髓核细胞中诱导型一氧化氮合酶和基质金属蛋白酶表达的影响

目的 探讨乌司他丁对白介素-1β(interleukin-1β,IL-1β)诱导的兔椎间盘髓核细胞中诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)、基质金属蛋白酶2(matrix metalloproteinase 2,MMP-2)和MMP-3表达的影响.方法 用酶消化法...

Effect of ulinastatin on expression of matrix metalloproteinase and nitric oxide synthase in nucleus pulposus cells of rabbits

Objective To observe the effect of ulinastatin on expression of inducible nitric oxide synthase（iNOS）,matrix metalloproteinase 2（MMP-2） and MMP-3 in degenerative nucleus pulposus cells of rabbit induced by interleukin-1β（IL-1β）.Methods Nucleus pulposus cells of rabbit collected and cultured by enzyme digestion in vitro were divided into 6 groups randomly： normal control group,ulinastatin control group,IL induced group and 3 inhibited gourps with ulinastatin at different concentration.After 48 h of intervention,nucleus pulposus cells were collected for detection of the content of iNOS with spectrophotometric method and the expression of MMP-2、MMP-3 by immunohistochemistry staining.Results Compared with the IL induced group,the content of iNOS in each ulinastatin inhibited group was decreased,most significantly in the 320 U/mL inhibited group,the differences were statistically significant（P0.01）;the expression of MMP-2,MMP-3 proteins in nucleus pulposus cells were decreased in the ulinastatin inhibited groups,the difference was statistically significant（P0.01）,and the decrease was in inverse proportion to ulinastatin concentration.Conclusion Ulinastatin in a certain concentration can inhibit the increased expression of MMP-2,MMP-3 and iNOS effectively and reduce the degradation of proteoglycan and collagen in nucleus pulposus cells induced by IL-1β in vitro.