滋肾通络方含药血清对高糖诱导的大鼠系膜细胞增殖的影响

目的：从细胞水平观察滋肾通络方含药血清对体外培养的大鼠肾小球系膜细胞(Glomerular Mesangial Cells，GMCs)增殖的影响，探讨其在糖尿病肾病(Diabetic Nephropathy，DN)防治中的可能机制。方法：体外培养培养大鼠肾小球系膜细胞株，利用噻唑蓝(3-［4，5-dimethylthylthiazol-2-yl］-2，5 diphenyltetrazolium broide，MTT)比色法动态观察各组含药血清作用下各组GMC不同时间点的增殖情况。结果：与正常组相比，高糖+LPS能明显刺激体外培养的GMC增殖，而与模型组相比，滋肾通络方含药血清能明显抑制GMC增殖。结论：高糖+LPS可促进GMC增殖，滋肾通络方含药血清能明显抑制高糖+LPS条件下GMC增殖，从而发挥预防和治疗肾小球硬化，进而延缓DN的进展。

Effects of Zishentongluo Decoction on the Proliferation of Glomerular Mesangial Cells Cultured in High Glucose

To observe the effects of drug containing serum of Zishentongluo Decoction(ZSTL) on glomerular mesangial cell (GMC) proliferationon in vitro under high glucose to explore its significance in the prevention and treatment of diabetic nephropathy (DN).Methods:The thiazolyl blue tetrazolium bromide (MTT) colorimetric method was used to observe the glomerular mesangial cell proliferation in different time.Results:Compared with the normal group, vitro cultured glomerular mesangial cells obviously proliferated under the stimulation of high glucose and LPS. Compared with the model group, the drug containing serum of Zishentongluo Decoction(ZSTL) could inhibit proliferation of GMCs.Conclusion:Vitro cultured glomerular mesangial cells could obviously proliferate under the stimulation of high glucose and LPS, and drug containing serum of Zishentongluo Decoction(ZSTL) can inhibit the proliferation of GMCs which could be helpful for preventing the progression of diabetic nephropathy.