GC-MS结合化学计量学解析法分析臭冷杉针叶挥发油成分

目的 分析臭冷杉Abies nephrolepis针叶挥发油中化学成分。方法 利用GC-MS联用技术进行分离检测，通过直观推导式演进特征投影（HELP）法对重叠峰进行解析，并结合程序升温保留指数（PTRI）进行辅助定性分析，采用峰面积归一化法对各组分进行定量分析。结果 共分辨出72个色谱峰，鉴定了其中63个组分，占臭冷杉挥发油化学成分总量的99.31%，其主要成分为醋酸龙脑酯、龙脑、α-红没药醇、柠檬烯等。结论 HELP法结合PTRI能够提高质谱定性的准确性，更全面、准确地反映臭冷杉针叶挥发油的化学成分。

Analysis on essential oil from needles of Abies nephrolepis by GC-MS combining with chemometric resolution method

Objective To optimize the preparation technology of recombinant hirudin-2 (rHV2) nanoparticles for nasal drug delivery. Methods Nanoparticles were prepared using chitosan (CS) and sodium tripolyphosphate (TPP) by complex coacervation. Taking the rHV2 encapsulation efficiency (EE) and drug loading as the evaluation indexes, the influencing factors and technological parameters in the preparation process were investigated by single factor and orthogonal tests. Results The results proved that not only the CS pH value, CS types, and the final proportion of CS to polyphosphate quality, but also the stirring speed in preparation had noticeable effect on the EE and drug loading of rHV2 nanoparticles. The EE and drug loading of optimized formulation could reach over 70% and 8%, repectively. Conclusion The optimized technology is stable and reliable, and could be used for the preparation of rHV2 nasal nanoparticles.