| 灰毡毛忍冬主栽品种的遗传多样性及其亲缘关系分析 |
| |
| 引用本文： | 陈大霞,张雪,李隆云. 灰毡毛忍冬主栽品种的遗传多样性及其亲缘关系分析[J]. 中国中药杂志, 2012, 37(13): 1912-1916 |
| |
| 作者姓名： | 陈大霞 张雪 李隆云 |
| |
| 作者单位： | 重庆市中药研究院中药种植研究所重庆市中药良种选育与评价工程技术研究中心重庆市银花工程技术研究中心,重庆,400065 |
| |
| 基金项目： | 国家"十一五"科技支撑计划项目(2006BAI06A12);重庆市自然科学基金项目(CSTC2009BB5395);重庆市重大科技攻关项目(CSTC2009AA5052);重庆市重大科技专项(CSTC2009AA5009) |
| |
| 摘 要： | 目的:研究灰毡毛忍冬主栽品种的遗传多样性及其亲缘关系.方法:以灰毡毛忍冬的5个主栽品种为试材,采用ISSR和SRAP分子标记技术,利用Treeconw软件分析处理数据,UPGMA法聚类,构建亲缘关系系统图.结果:20条ISSR引物共得到186条扩增条带,其中有103条呈现多态性,占54.63％,遗传距离0.058 4～0.230 8,平均值为0.1902.58个SRAP引物组合共得到591条扩增条带,其中有347条呈现多态性,占55.46％,遗传距离在0.1071～0.261 1,平均值为0.212 2.2种标记均表明灰毡毛忍冬主栽品种的遗传多样性仅居中等水平.2种标记系统得到了相似但并不完全相同的聚类图.结论:灰毡毛忍冬主栽品种有中等水平的遗传多样性,ISSR与SRAP标记均适用于其遗传多样性的分析.
|
| 关 键 词： | 灰毡毛忍冬 ISSR SRAP 遗传多样性 亲缘关系 品种 |
| 收稿时间： | 2011-12-23 |
Analysis of genetic diversity and genetic relationships of Lonicera macranthoides cultivars |
| |
| CHEN Daxi,ZHANG Xue and LI Longyun. Analysis of genetic diversity and genetic relationships of Lonicera macranthoides cultivars[J]. China Journal of Chinese Materia Medica, 2012, 37(13): 1912-1916 |
| |
| Authors: | CHEN Daxi ZHANG Xue LI Longyun |
| |
| Affiliation: | Chongqing Engineering Research Center of Lonicea Flos, Chongqing Research Center of Techniques and Engineering for Chinese Materia Medica Fine Variety Breeding and Evaluation, Institute of Material Medical Planting, Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China;Chongqing Engineering Research Center of Lonicea Flos, Chongqing Research Center of Techniques and Engineering for Chinese Materia Medica Fine Variety Breeding and Evaluation, Institute of Material Medical Planting, Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China;Chongqing Engineering Research Center of Lonicea Flos, Chongqing Research Center of Techniques and Engineering for Chinese Materia Medica Fine Variety Breeding and Evaluation, Institute of Material Medical Planting, Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China |
| |
| Abstract: | Objective: To study genetic diversity and genetic relationships among Lonicera macranthoides cultivars. Method: Five cultivars were estimated by ISSR and SRAP. The data of amplified bands were analyzed by Treeconw software. The system diagram of genetic relationship was built by UPGMA. Result: Twenty ISSR primers amplified 186 bands with 103(54.63%) polymorphic bands and 58 SRAP primer combinations amplified 591 bands with 347(55.46%) polymorphic bands. Genetic distance ranges were 0.058 4-0.230 8 (by ISSRs) and 0.107 1-0.261 1(by SRAPs). Both ISSR and SRAP analyses revealed a middle level of genetic diversity in L. macranthoides cultivars. The dendrograms based on SRAP and ISSR markers were not all the same. Conclusion: The genetic diversity of L. macranthoides cultivars is middle. ISSR and SRAP markers can be effectively applied to genetic analysis in L. macranthoides cultivars. |
| |
| Keywords: | Lonicera macranthoides ISSR SRAP genetic diversity genetic relationship breeding |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
