| RP-HPLC法测定定坤丸中芍药苷的含量 |
| |
| 引用本文： | 王莉,崔明,文艳秋,徐新盛,毕开顺. RP-HPLC法测定定坤丸中芍药苷的含量[J]. 西北药学杂志, 2005, 20(5): 202-203 |
| |
| 作者姓名： | 王莉 崔明 文艳秋 徐新盛 毕开顺 |
| |
| 作者单位： | 1. 沈阳药科大学药学院,辽宁,沈阳,110016
2. 北京中丰天恒医药技术开发有限公司,北京,100012 |
| |
| 摘 要： | 目的建立定坤丸中芍药苷含量的测定方法。方法采用反相高效液相色谱法,Kromasil C18柱(4.6mm×250mm,5μm),以乙腈-水梯度系统为流动相,检测波长230nm。结果芍药苷在3.664~36.64mg·L-1范围内线性关系良好,r=0.9997(n=5);平均回收率为99.6%(n=9),RSD为1.0%。结论本法简便、准确,重现性好,为定坤丸的质量评价提供了依据。
|
| 关 键 词： | 定坤丸 芍药苷 反相高效液相色谱法 |
| 文章编号： | 1004-2407（2005）05-0202-02 |
| 收稿时间： | 2005-03-25 |
| 修稿时间： | 2005-03-25 |
Determination of the content of paeoniflorin in Dingkun pills by RP-HPLC |
| |
| WANG Li,CUI Ming,WEN Yan-qiu,XU Xin-sheng,BI Kai-shun. Determination of the content of paeoniflorin in Dingkun pills by RP-HPLC[J]. Northwest Pharmaceutical Journal, 2005, 20(5): 202-203 |
| |
| Authors: | WANG Li CUI Ming WEN Yan-qiu XU Xin-sheng BI Kai-shun |
| |
| Affiliation: | WANG Li~1,CUI Ming~1,WEN Yan-qiu~2,XU Xin-sheng~2,BI Kai-shun~1 |
| |
| Abstract: | Objective A high-performance liquid chromatographic method was developed for the determination of the content of paeoniflorin in Dingkun pills. Method A Kromasil C_(18) column(4.6 mm×250 mm ,5 μm) was used, with the mobile phase consisting of acetonitrile-H_2O binary system. The detection wavelength was 230 nm. Results The linear range of paeoniflorin was 3.664～36.64 mg·L~(-1), r = 0.999 7(n = 5); the average recovery was 99.6%(n = 9), RSD was 1.0%. Conclusion The method is simple and can be used to determine paeoniflorin in Dingkun pills with satisfactory accuracy and reproducibility. |
| |
| Keywords: | Dingkunwan Paeoniflorin RP-HPLC |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
