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Different genetic backgrounds for malnutrition-related diabetes and Type 1 (insulin-dependent) diabetes mellitus in South Indians
Authors:Dr. C. B. Sanjeevi  V. Seshiah  E. Möller  O. Olerup
Affiliation:(1) Center for BioTechnology, Karolinska Institute, NOVUM, Sweden;(2) Department of Clinical Immunology, Karolinska Institute at Huddinge Hospital, Huddinge, Sweden;(3) Department of Diabetology, Madras Medical College and Government General Hospital, Park Town, Madras, India;(4) Department of Molecular Diabetes Research, Wallenberg Laboratory II, Box 7031, S-220 07 Lund, Sweden
Abstract:Summary HLA-DRB, -DQA and -DQB genes were studied in ten South Indian malnutrition-related diabetic patients, ten Type 1 (insulin-dependent) diabetic patients and 45 control subjects, by TaqI restriction fragment length polymorphism analysis. The DR7,DQw9 haplotype was found to be frequent in patients with malnutrition-related diabetes (p<0.01). The DRw17,DQw2 haplotype was overrepresented in the patients with Type 1 diabetes compared to control subjects (p<0.05). In vitro amplification of the polymorphic second exon of DQB genes by the polymerase chain reaction technique was performed on DNA from 10 malnutrition-related diabetic patients, 10 Type 1 diabetic patients and 13 control subjects, as they belong to a new population. Hybridization with sequence-specific oligonucleotide probes for DQB1 alleles showed homozygosity of aspartic acid at position 57 in 7 of 10 malnutrition-related diabetic patients compared to 2 of 10 Type 1 diabetic (p<0.05) and 15 of 45 control subjects (p<0.05). Homozygosity of non-aspartic acid at position 57 was present in 7 of 10 Type 1 diabetic compared to 0 of 10 malnutrition-related diabetic patients (p<0.005) and 3 of 45 control subjects (p<0.05). This study has confirmed the association of DQB1 57 non-asp in South Indians with Type 1 diabetes. In addition, our data clearly show that the genetic background of malnutrition-related diabetes mellitus is different from that of Type 1 diabetes.
Keywords:Type 1 (insulin-dependent) diabetes mellitus  malnutrition-related diabetes mellitus  South Indians  HLA-DQ  genetic susceptibility
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