大鼠肝脏缺血再灌注损伤早期载脂蛋白M的表达

目的 探讨大鼠肝缺血再灌注损伤早期肝内载脂蛋白M mRNA(apoM mRNA)及血浆apoM的表达。方法 建立大鼠肝脏缺血再灌注损伤模型。健康雄性SD大鼠40只随机分成5组，每组8只：假手术组(对照组)；IR1组(灌注0．5h)；[R2组(灌注1．0h)；IR3组(灌注2．0h)；[R4组(灌注3．0h)。缺血再灌注组的缺血时间统一为1．0h。检测血浆谷丙转氨酶水平(ALT)、肝组织病理变化、血浆apoM蛋白及肝组织apoM mRNA。结果 血浆ALT的水平随着灌注时间的延长而升高，肝组织损伤随着灌注时间的延长而逐渐加重。肝组织apoM mRNA的表达则先有一过性下降(灌注0．5h组)，此后随着灌注时间的延长其表达明显增强。血浆apoM蛋白有相同的变化趋势，但其表达在灌注2．0h才有上升。结论 在肝缺血再灌注损伤过程中，肝脏apoM mRNA的表达和血浆蛋白水平有迅速、明显的变化，提示apoM可能具有急性时相反应蛋白的特性。

Early apolipoprotein M expression in ischemia -reperfusion injury of rats

ObjectiveTo investigate the expression of hepatic apolipoprotein M (apoM) mRNA and serum apoM during early stage of hepatic ischemia reperfusion injury in rats. MethodsRat models of hepatic (ischemia)-reperfusion injury (IRI) were established. Forty healthy male SD rats were randomly divived into five groups(each group containing eight rats):Sham-operation group(control); IRI groups, animals (experienced) 1 hr ischemia and then followed by various reperfusion intervals(0.5,1.0,2.0 and 3.0 hrs, respectively). ResultsSerum alanine-aminotransferase (ALT) levels and the liver injury degree (histologically) were increased with the prolongation of hepatic reperfusion time. The apoMmRNA level in liver was markedly decreased in the group that had reperfusion for 0.5hr compared to the sham group. However, it increased gradually in groups that had reperfusion for 1hr to 3hrs. Serum apoM protein level showed a (similar) tendency with apoMnRNA, but it increased after reperfusion for 2 hrs. ConclusionsIt is concluded that apoM expression pattern shows rapid and significant changes during hepatic ischemia-reperfusion injury, which suggests that apoM might have the characteristic of acute phase reactive protein.