蕨麻正丁醇部位对EAHY926内皮细胞缺氧损伤的保护作用

【目的】探讨蕨麻正丁醇部位对内皮细胞缺氧损伤的保护作用。【方法】采用人脐静脉内皮细胞株(EAHY926)建立缺氧损伤实验模型,通过MTT法测定各实验组细胞代谢率;比色法测定乳酸脱氢酶(lactate dehydrogenase,LDH)、细胞内超氧化物歧化酶(superoxide dismutase,SOD)活性和NO浓度;放射免疫法测定内皮素-1(endothelin-1,ET-1)的活性。【结果】3.00 mg/ml、1.50 mg/ml和0.75 mg/ml蕨麻正丁醇部位均能显著减少缺氧损伤内皮细胞LDH的外漏量,并可显著提高细胞内SOD活性,增加NO分泌、减少ET-1的生成。【结论】蕨麻正丁醇部位对内皮细胞缺氧损伤具有显著的保护作用,其机制之一可能是清除缺氧导致的内皮细胞自由基堆积,减少ET-1的释放,增加NO分泌,从而减轻内皮细胞损伤。

Protective effect of n-butanol extract of Potentilla anserina L.on hypoxia injury in EA.hy926 endothelial cells

Objective ] To study on the protective effect of n-butanol extract of Potentilla anser/ne L. on EAHY926 endothelial cell injured by hypoxia. Methods] A model of hypoxia injury was established by using human umbilical vein endothelial cell line （EAHY926）. The metabolic ability of the cells was detected by MTT assay, the activities of lactate dehydrogenase （LDH）, nitrogen monoxidum （NO） and endothelin （ET-1） out of ceils and superoxide dismutase （SOD） in cells were measured by biochemistry approaches. Results] 3.00 mg/ml, 1.50 mg/ml and 0.75 mg/ml of n-butanol extract of Potentilla anserine L. could significantly reduce the release of LDH and ET-1, increase the release of NO and increase activity of SOD in cells during hypoxia injury. Conclusion] Potentilla anser/na L. showed a remarkably protective effect on hypoxia injury in endothelial ceils.