HBV感染产妇血清和乳汁中HBV-M与HBV-DNA检测

目的检测HBV感染产妇乳汁中HBV-M和HBV-DNA,以评价母乳喂养的安全性。方法采用ELISA静置式与振动式孵育法定性检测乳汁HBsAg,同时采用荧光定量PCR法检测血清和乳汁HBV-DNA。结果 144例产妇血清HBV-DNA阳性率52.1%（75/144）,乳汁阳性率34.7%（50/144）,乳汁HBsAg静置式孵育法阳性66例（45.8%）,振动式孵育为75例（52.1%）。结论应尽可能同时检测乳汁的HBsAg和HBV-DNA以提高母乳喂养的安全性。无HBV-DNA检测条件的实验室在检测乳汁中HBsAg时应采用振动式孵育以提高阳性检出率。

Detection of HBV-M and HBV-DND in the serum and lac feminium of HBV-infected pureperas

Objective To detect HBV-M and HBV-DND in the lac feminium of HBV-infected pureperas in order to evaluate the safety of breast feeding.Methods ELISA standing type and oscillator type brooding-methods were adopted to detect HBsAg in the lac feminium qualitivly while fluorescent quantitation PCR method was applied to detect HBV-DNA in the serum and lac feminium.Resluts The positive rate of HBV-DNA was 52.1%(75/144)in the serum of 144 HBV-infected puerperas and 34.7%(50/144)in the lac feminium.Moreover,66(45.8%)cases of positive HBsAg were detected by the standing type of brooding-method and 75(52.1%) by the oscillator type brooding-method.Conclusions HBsAg and HBV-DNA in the lac feminium should be detected simultaneously to improve the safety of breast feeding.Oscillator type brooding-method should be adopted to detect lac feminium to enhance the positive rate of HBsAg in laboratories unable to detect HBV-DNA.