| 比较基因组杂交中的自动图像分析关键技术 |
| |
| 引用本文： | 倪江,季梁,王明荣. 比较基因组杂交中的自动图像分析关键技术[J]. 生物医学工程学杂志, 2001, 18(4): 620-623 |
| |
| 作者姓名： | 倪江 季梁 王明荣 |
| |
| 作者单位： | 1. 清华大学,自动化系模式识别与人工智能国家重点实验室,北京,100084
2. 中国医学科学院中国协和医科大学,肿瘤研究所细胞生物室分子肿瘤学国家重点实验室,北京,100021 |
| |
| 基金项目： | 国家自然科学基金资助项目（39770227） |
| |
| 摘 要： | 比较基因组杂交是细胞遗传学的一种新方法，用于实体肿瘤染色体研究。图像分析是比较基因组杂交的重要部分，旨在从实验测得的荧光图像中，分析得到待测肿瘤组织拷贝数的情况，从而做出病情诊断，或用于肿瘤遗传学研究。本文综述了比较基因组杂交中的图像分析中的关键技术，着重于本底校正、染色体分割、求染色体中轴、核型分析和求比率这几个关键步骤，并讨论了未来的发展方向。
|
| 关 键 词： | 比较基因组杂交 图像分析 细胞遗传学 染色体 肿瘤学 |
Key Technologies in Image Analysis of Comparative Genomic Hybridization |
| |
| Ni Jiang Ji Liang Wang Mingrong. Key Technologies in Image Analysis of Comparative Genomic Hybridization[J]. Journal of biomedical engineering, 2001, 18(4): 620-623 |
| |
| Authors: | Ni Jiang Ji Liang Wang Mingrong |
| |
| Affiliation: | State Key Laboratory of Intelligent Technology and Systems of China, Department of Automation, Tsinghua University, Beijing 100084. |
| |
| Abstract: | Comparative genomic hybridization has become a new technique in molecular cytogenetics, and it has found significant applications in tumor pathology. Image analysis is an important part of comparative genomic hybridization. It analyzes the fluorescent images by many steps and finally determines whether there is any abnormality in the copy number of the test tumor tissue. This paper expatiates on the key steps in the image analysis of comparative genomic hybridization, including background correction, chromosome segmentation, chromosome axis determination, karyotyping and determining ratio profile. The future trend is also discussed. |
| |
| Keywords: | Comparative genomic hybridization Image analysis Cytogenetics Chromosome Oncology |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
