采集后6小时与72小时制备的冰冻单采血小板质量研究

目的通过比较采集后6h和72h制备的冰冻单采血小板制品的多项质量参数，为冰冻单采血小板的制备标准提供依据。方法以美国产Trima血细胞分离机配套全密闭7d保存袋采集的单采血小板，分别于采集后6h和72h制备为冰冻血小板制品，1周后复苏留样，分别检测血小板计数（PLT，MPV，PCT，PDW）、血小板粘附性、血小板P选择蛋白、PF3A、PF4、pH值、血块收缩试验（血浆法）。结果采集后6h和72h制备的冰冻单采血小板，两者相比差异无统计学意义（t〈0．01，P〉0．05）。单采血小板在不同保存条件下，PF3A均保持了良好的PF3活性，单采血小板在常规或冰冻保存条件下，PF4及P选择蛋白的表达均明显增加，采集后6h和72h制备的冰冻单采血小板相比，差异无统计学意义（t〈0．01，P〉0．05）；单采血小板在常规保存3d内，粘附功能和血块收缩试验没有明显变化，而冰冻保存的单采血小板这两项指标呈明显减退现象，血小板的活力下降明显。结论以美国产Trima血细胞分离机配套全密闭7d保存袋采集的单采血小板，分别于采集后6h和72h制备的冰冻血小板制品，两者的体外指标分析结果相比差异无显著性，且都能有效的改善和控制急性大出血患者的出血倾向，用于抢救急性大出血患者疗效是可靠的。

A comparative study on qualities of frozen platelets prepared 6 h and 72 h after collection

Objective To offer evidence for preparation criteria of frozen platelet preparation by means of comparison of a number of quality parameters between the frozen platelets prepared at 6 hour and 72 hour time point after collection.Methods Frozen platelet preparation was made 6 h and 72 h after collection of blood platelets gathered in bags by Trima Blood Corpuscle Machine.A week later, the frozen platelet preparation was dissolved and sampled for detection of PLT,MPV,PCT,PDW, platelet adhesiveness,P-selectin,PF3A,PF4,pH value,blood clot contractibility(clot retraction test).Results There were no significant differences in a series of quality parameters between the frozen platelets prepared at 6 hours and 72 hours after collection.Under the different conservation conditions.PF3A kept available activity.When the blood platelets were conserved under the conditions at22℃or at-80℃,the expressions of PF3A and p-Selection were increased,there was no difference of PF3A and p-Selection expression between the frozen platelets prepared at 6 hours and 72 hours after collection.During three days of routine conservation,no change appeared in the conglutinate function and clot contractibility;however,the above parameters were significantly decreased,and the activity of platelets was markedly datined under congealing.Conclusion There are no significant differences in a series of quality parameters between the frozen platelet preparation prepared with gathered blood by Trima Blood Corpuscle Machine at 6 hours and 72 hours after collection,and both of the frozen platelet preparation can effectively improve or control the hemorrhagic tendency of patients suffered urgent massive hemorrhage.