| Image Analysis for Degradation of DNA in Retinal Nuclei of Rat after Death |
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| 作者姓名： | 陈晓瑞 易少华 刘良 |
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| 作者单位： | Facultry of Forensic Medicine Tongji Medical College Huazhong University of Science and Technology，Facultry of Forensic Medicine Tongji Medical College Huazhong University of Science and Technology，Facultry of Forensic Medicine Tongji Medical College Huazhong University of Science and Technology，Wuhan 430030 China，Wuhan 430030 China，Wuhan 430030 China |
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| 摘 要： | The changes of retinal nuclear DNA content in rats after death was detected and the rela-tionship between degradation of retinal nuclear DNA and postmortem interval (PMI) was analyzed. Ninety healthy adult SD rats, female, weighing 250±10 g, were randomly divided into 15 groups. At 20 ℃, the retinal cells were withdrawn every 2 h within 0 to 28 h after death and stained with Feul-gen-Vans. Index of density (ID), integral absorbance (IA) and average absorbance (AA) in retinal nu-cleus were analyzed by image analysis system. And the obtained data were subjected to linear regres-sion analysis by using SPSS12.0 software. The results showed that in retinal nucleus, AA and IA were gradually declined with the prolongation of PMI, while ID had an increased tendency. Within 28 h after PMI, the regression equations were as follows: YAA=-0.009XAA 0.590 (R2=0.949), YIA=-0.097XIA 18.903 (R2=0.968), YID=0.122XID 2.246 (R2=0.951). It was concluded that retinal nuclear DNA after death in rats was degraded gradually and had a good correlation with PMI.
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| 关 键 词： | 影像分析 死亡 视网膜 老鼠 动物实验 |
| 收稿时间： | 2006-10-10 |
Image analysis for degradation of DNA in retinal nuclei of rat after death |
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| Chen?Xiaorui,Yi?Shaohua,Liu?Liang.Image Analysis for Degradation of DNA in Retinal Nuclei of Rat after Death[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2007,27(1):24-26. |
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| Authors: | Chen Xiaorui Yi Shaohua Liu Liang |
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| Institution: | Facultry of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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| Abstract: | Summary The changes of retinal nuclear DNA content in rats after death was detected and the relationship between degradation of retinal
nuclear DNA and postmortem interval (PMI) was analyzed. Ninety healthy adult SD rats, female, weighing 250±10 g, were randomly
divided into 15 groups. At 20 °C, the retinal cells were withdrawn every 2 h within 0 to 28 h after death and stained with
Feulgen-Vans. Index of density (ID), integral absorbance (IA) and average absorbance (AA) in retinal nucleus were analyzed by image analysis system. And the obtained data were subjected to linear regression analysis
by using SPSS12.0 software. The results showed that in retinal nucleus, AA and IA were gradually declined with the prolongation of PMI, while ID had an increased tendency. Within 28 h after PMI, the regression
equations were as follows: YAA
=−0.009XAA
+0.590 (R2=0.949), YIA
=−0.097XIA
+18.903 (R2=0.968), YID=0.122XID+2.246 (R2=0.951). It was concluded that retinal nuclear DNA after death in rats was degraded gradually and had a good correlation with
PMI.
CHEN Xiaorui, male, born in 1964, Associate Professor, M.D., Ph.D.
This project was supported by a grant from Hubei Provincial Natural Sciences Foundation of China (No. 2004 ABA200). |
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| Keywords: | postmortem interval DNA retinal image analysis system |
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