胃饲乙醇预处理诱导肝脏缺血耐受性的可行性探讨

目的探讨胃饲乙醇预处理对肝脏缺血再灌流损伤的影响,并初步评价其预处理的可行性。方法40%乙醇胃饲Wistar大鼠。分组:①大鼠36只,随机分6组:A组8g/kg、B组7g/kg、C组6g/kg、D组5g/kg、E组4g/kg、正常组0g/kg;以中毒症状及肝组织病理为指标,判定大鼠乙醇急性中毒剂量。②大鼠78只,随机分为4组:正常对照组(N)、单纯乙醇组(E)、单纯缺血组(ISCH)、胃饲乙醇预处理组(EPC);采用尾叶转流下的肝缺血模型,于再灌流3、6、12、24h留取标本。结果急性胃饲乙醇≤5g/kg预处理后,动物中毒症状轻,无死亡;乙醇预处理可以在一定程度上减轻肝脏90min的缺血再灌流损伤。结论适当剂量的乙醇胃饲预处理是一种安全的预处理措施,有望成为增强肝脏对缺血再灌流损伤耐受性的一种较好的预处理方式。

The feasibility of enhancing the tolerance of the liver to ischmia-reperfusion injuries in rats by ethanol

Objective To investigate the feasibility and effica cy of the ethanol precondition. Study was designed to confirm the proper range of the ethanol according to the toxicity and mortality, and then evaluate the possibility of application of the ethanol pretreatment. Methods (1) 36 male adult wistar rats pretreated with 40% ethanol were randomly divided into six groups according to different dosage. Group A( 8 g/kg), Group B (7 g/kg), Group C (6 g/kg), Group D (5 g/kg), Group E (4 g/kg), and nor mal control group (0 g/kg). The safe dosage range of ethanol in rats were defermined observation of the symptoms after ethanol administration and pathological chang es after 24 h. (2) This experiment were set based on the results of experiment a s 78 Wistar rats were randomly divided into 4 groups: normal control group (N), Ethanol group (E), ischemia group (ISCH), ethanol preconditioning group (EPC). In each group, the specimens were harvested at 3, 6, 12 and 24 h after reperfusion. Results Ethanol below the dose of 5 g/kg is safe to the rat and can reduce the 90 minutes of IR injuries to the liver. Conclusions Proper dose of ethanol gavage to the rat is safe and may be a new method of pretreatment to enhance the toler ance of rat liver to the IR injuries.