姜黄素对人骨肉瘤细胞株/阿霉素多药耐药逆转作用的实验研究

目的观察姜黄素对多药耐受糖蛋白(P-gp)介导的人骨肉瘤细胞株/阿霉素(U-2OS/ADM)细胞多药耐药(MDR)的逆转作用。方法以阿霉素为诱导药物,人骨肉瘤细胞系U-2OS为诱导对象,采用大剂量冲击法建立人骨肉瘤多药耐药细胞系模型(U-2OS/ADM),采用MTT法检测姜黄素作用于U-2OS/ADM细胞前后对化疗药物的逆转;流式细胞仪检测细胞内罗丹明-123积聚和外排的影响。结果 MTT显示姜黄素20μmol/L能增加不同浓度阿霉素对U-2OS/ADM细胞的抑制作用(P<0.01),且姜黄素对阿霉素为2.0μg/ml时的U-2OS/ADM细胞抑制作用增加幅度达49%。FCM结果显示,姜黄素能增加阿霉素对U-2OS/ADM的细胞毒性作用,且呈剂量依赖关系。结论姜黄素可有效逆转U-2OS/ADM细胞的多药耐药现象的作用。

Empirical study of reverse effect on mediated multidrug resistance of U-20S/ADM cells with curcumin in vitro

Objective To investigate the reversion of P-gp mediated multidrug resistance of U-2OS /ADM cells with curcumin in vitro.Methods With doxorubicin as induction drugs,human osteosarcoma cell line U-2OS was induced object,multi-drug resistant of human osteosarcoma cell line model(U-2OS /ADM) were established by high-dose method,The before and after reversal efficacy of curcumin for U-2OS /ADM cells to chemotherapeutic drugs were measured by MTT assay.The effects of curcumin on Rh-123 uptake and efflux were analyzed by flow cytometer.Results(1) MTT demonstrates curcumin(20μmol /L) can increase the cytotoxicity of Adriamycin to U-2OS /ADM cells.(2) The result of FCM shows that Curcumin can increase the accumulation of Rh-123 and increase the cytotoxicity of Adriamycin to U-2OS /ADM cells in a dose-dependent manner.Conclusion The reversal mechanism of curcumin was blocked the function of P-gp in U-2OS /ADM cellular membrane.