葡萄原花青素对乙醇诱发小鼠肝细胞Caspase-3和Bax蛋白异常表达的影响

目的研究葡萄原花青素(GPC)对乙醇诱发小鼠肝细胞Caspase-3和Bax蛋白异常表达的影响。方法将小鼠随机分为正常对照组、乙醇对照组和各剂量GPC组,除正常对照组外,每组小鼠均每天经口灌胃乙醇4g/kg,低、中、高剂量GPC组同时分别给予GPC 50、100和200mg/kg,连续4周。取肝组织用MTT法检测各组小鼠的肝细胞增殖活性,用免疫组织化学法检测肝细胞Caspase-3和Bax蛋白的表达水平。结果高剂量GPC组Caspase-3和Bax蛋白阳性表达率分别为19.08%和14.06%,乙醇对照组则为51.78%和58.08%,差异有显著性(P<0.05)。高、中剂量GPC组细胞增殖活性高于乙醇对照组(P<0.05)。结论 GPC可抑制乙醇诱发的肝细胞Caspase-3和Bax蛋白异常表达,对乙醇性肝损伤具有防护作用。

Effect of grape procyanidins on expressions of Caspase-3 and Bax in ethanol-induced liver injury in mice

Objective To study the effect of grape procyanidins(GPC) on the expressions of Caspase-3 and Bax in the liver of mice with ethanol-induced liver injury.Method The mice were orally given at the different doses of GPC(50,100 and 200 mg /kg) and 4g /kg ethanol,and killed after 4 weeks.The proliferation activity of the hepatic cells was determined by MTT assay,the levels of Caspase-3 and Bax protein expression in the cells were measured by immunohistochemistry method.Results The expression level of Caspase-3 and Bax protein in high dose group were 19.08% and 14.06%,respectively,and those of the ethanol-injured group were 51.78% and 58.08%.The differences between the two groups were significant(P < 0.05).The cell proliferation activity of high dose and medium dose group was higher than the cell proliferation activity of ethanol-injured group(P < 0.05).Conclusion GPC could suppressing the expression of Caspase-3 and Bax in the liver of mice with ethanol-induced liver injury.