全反式维甲酸对体外培养的人Tenon囊成纤维细胞增殖及凋亡的影响

目的：观察全反式维甲酸(ATRA)对体外培养的人Tenon囊成纤维细胞（HTCFs）增殖及凋亡的影响。方法：体外培养HTCFs，采用流式细胞仪检测细胞周期改变、细胞凋亡率、抗凋亡基因bcl-2的水平，琼脂糖凝胶电泳检测细胞DNA，研究不同浓度的ATRA对体外培养的HTCFs增殖及凋亡的影响，同时与丝裂霉素C（MMC）作比较。结果：用ATRA和MMC处理后，HTCFs细胞周期的分布发生了明显的变化，表现为G⁰/G¹期细胞数增多，S期细胞数减少；1×10_-9mol/L的ATRA即可诱导细胞的凋亡，随着浓度的增加，凋亡率显著增高；ATRA组bcl-2的水平比空白对照组大约低了一倍；DNA琼脂糖凝胶电泳结果显示：ATRA组为呈一定间隔的DNA梯形条带，MMC组呈现弥漫的片状图谱，无明显的条带。结论：ATRA可有效抑制体外培养的HTCFs的增殖，但与MMC的作用方式不同。MMC主要造成细胞坏死，而ATRA则主要诱导细胞凋亡，无明显细胞毒作用。

Effect of all trans-retinoic acid on the proliferation and apoptosis of human Tenon's capsule fibroblasts in vitro

Objective: To study the effect of all trans-retinoic acid (ATRA) on the proliferation and apoptosis of human Tenon′s capsule fibroblasts(HTCFs) in vitro. Methods: Cell cycle distribution, the apoptotic rate and the expression of bcl-2 were determined by flow cytometry. Cell DNA was determined by agarose gel electrophoresis. Mitomycin C was used as a positive control. Results: Cell cycle distribution of HTCFs showed that cells of the G⁰/G¹ phase increased and of the S phase decreased after the treatment with ATRA and MMC. Cellular apoptosis was decreased by ATRA at a lower dose (1×10_-9mol/L), and the apoptotic rate was significantly increased in a dose-dependent manner. Expression of bcl-2 was significantly decreased by ATRA. A “ladder” strand of DNA was produced of the ATRA groups by DNA agarose gel electrophoresis. Conclusion: ATRA can significantly inhibit the proliferation of HTCFs and induce the apoptosis of HTCFs without apparent cytotoxicity.