儿茶素和漆黄素对柠檬酸铁诱导大鼠原代肝细胞损伤的保护作用

目的　分别从中药苞蔷薇根和叶中提取获得 (+ ) 儿茶素 (CAT )和漆黄素 (FIS)两种黄酮 ,研究其对柠檬酸铁致肝细胞铁超载的药理作用。方法　原代大鼠肝细胞加入 50μmol·L- 1柠檬酸铁诱导肝细胞铁超载。致损伤前加入CAT及FIS或损伤后 2 4h ,加入CAT及FIS ,继续培养 2 4h后 ,测定培养液中的超氧化物歧化酶 (SOD )、乳酸脱氢酶(LDH )、谷草转氨酶 (AST)、谷丙转氨酶 (ALT)活性和白蛋白 (ALB)含量。培养 48h后 ,用MTT法测肝细胞的存活率。结果　在致损伤前加入CAT或FIS ,仅见CAT 5× 10 - 5mol·L- 1和FIS 5× 10 - 5 mol·L- 1对大鼠原代肝细胞的恢复作用与模型对照组相比差异有显著性 (P <0 0 5或P <0 0 1)。在致损伤后加入CAT或FIS ,可见CAT 5× 10 - 5 、5× 10 - 6 、5× 10 - 7mol·L- 1组 ,FIS 5× 10 - 5 、5× 10 - 6 、5×10 - 7mol·L- 1组均可不同程度的提高培养液中的SOD活性 ,降低LDH水平 ,抑制ALT、AST的释放 ,提高ALB的含量 ,提高培养液中肝细胞的存活率 ,与模型对照组比 ,差异有显著性 (P <0 0 1,P <0 0 5)。FIS 5× 10 - 5 mol·L- 1组与正常对照组相比 ,对于提高SOD活性、ALB含量 ,差异无显著性 (P >0 0 5)。结论　CAT、FIS对柠檬酸铁所致大鼠原代肝细胞的损伤具有保护作用

Protection of iron-induced toxicity in rat hepatocyte culture by (+)-catechin and fisetin

AIM To investigate the protective effects of CAT or F IS from Rosa bracteata Wendl on the iron-loaded rat hepatocytes. METHODS Iron over-loaded model was induced by addition of 50 μmol ·L -1 iron citrate to the rat hepatocytes. At the meantime or 24 h after iron treatment, CAT, FIS or Dereroxamine was added to the culture medium . At 24 h after the drug treatment, the activities of SOD, LDH, ALT and AST of the in rat hepatocyte cultures were measured. At 48 h after the drug treatment, the survival rate of hepatocytes was measured. RESULTS CAT and FIS h ave less effect on the rat hepatocytes treated with the iron and drug together, while the group of CAT 5×10 -5 、5×10 -6 、5×10 -7 mol·L -1 ,FIS 5×10 -5 , 5×10 -6 , 5×10 -7 mol·L -1 DFO treated w ith iron first could raise the content of SOD, restrain the release of AST and A LT, lower the level of LDH and the content of ALB on those treated with the iron first. The survival rate of hepatocytes also increased. CONCLUSION CAT and FIS have a good protection against the iron toxicity in rat hepatocyte c ultures.