外翻肠囊模型结合光纤传感过程分析蒜氨酸在家兔肠道的吸收

目的： 考察大蒜提取物蒜氨酸在家兔肠道内的吸收特性，为新药研究提供实验数据。 方法： 采用家兔，利用外翻肠囊模型与光纤传感过程分析系统结合，实时原位测定蒜氨酸在家兔肠道内的浓度变化。研究蒜氨酸在家兔十二指肠、空肠、回肠、结肠的4个肠段，高、中、低3个浓度的吸收，每个肠段的每个浓度进行6样本（6只家兔，共计18只家兔）分析，每个肠段接触蒜氨酸的浓度根据半数致死量确定，配制蒜氨酸溶液的质量浓度分别为（700，50，30 mg·L^-1），于光纤传感过程分析仪上进行检测。 结果： 家兔不同肠段对蒜氨酸的吸收能力大小：回肠>空肠>结肠>十二指肠；不同浓度下各肠段的吸收数A_n值空肠分别为（2.59±0.21，5.40±0.11，3.81±0.27）；回肠分别为（5.25±0.07，3.02±0.19，4.09±0.18），各浓度A_n值均大于1.15，十二指肠分别为（0，0.95±0.16，1.09±0.28）；结肠分别为（0.50±0.13，0.90±0.22，1.08±0.24），各浓度A_n值均<1.15。各浓度在4个肠段的K_e值除十二指肠段外（0，0.007±0.02，0.004±0.09）其余肠段的K_e值都<0.01 cm·min^-1。 结论： 利用光纤传感系统实时测定蒜氨酸原料药在肠道的浓度变化，从而研究其在各肠段的吸收，结果表明蒜氨酸在除十二指肠段外其余肠段的渗透性很好。

Optical Fiber Sensing Process Analysis Combined Study on Absorption of Alliin in Intestine of Rabbits through Optical Fiber Sensing Process Analysis Combinedwith Everted Gut Sac Model

Objective: To investigate the intestinal absorption characteristics of alliin extract from garlic in the rabbit, and provide experimental data for pre-formulation study. Method: Using optical fiber sensing process analysis system combined with everted gut sac model and real-time and in situ determination of the concentration of alliin in the rabbit intestine, the absorption of different concentrations (70, 50, 30 mg·L^-1)was observed in different bowels (n=6). Result: The absorption capacity of alliin in different bowels was as follows: jejunum> ileum > colon > duodenum; different concentrations of intestine jejunum absorption number A_n values were (2.59 ± 0.21, 5.40 ± 0.11, 3.81 ± 0.27); ileum were (5.25 ± 0.07, 3.02 ± 0.19, 4.09 ± 0.18) were greater than the value of A_n 1.15, duodenum were (0, 0.95 ± 0.16,1.09 ± 0.28); colon were (0.50 ± 0.13, 0.90 ± 0.22, 1.08 ± 0.24) for each concentration A_n values were less than 1.15. K_e values for each concentration except duodenum (0, 0.007 ± 0.22, 0.004 ± 0.29) others are greater than 0.01 cm·min^-1. Conclusion: Fiber Optic sensing system may used to determine the absorption of alliin(APIs) in the intestine in real-time, and the results show permeability of alliin in rabbit intestinal.