扶正活血清热方预防大鼠急性放射性肠炎机制分析

目的： 探讨扶正活血清热方有效预防大鼠急性放射性肠炎（acute radiation enteritis，ARE）的作用机制。 方法： 将48只成年健康Wistar雄性大鼠随机分为6组（8只/组），即空白对照组、模型组、地塞米松组、扶正活血清热方高、中、低剂量组。除空白对照组不给予处理外，模型组、地塞米松组和扶正活血清热方高、中、低剂量组大鼠于照射造模前分别给予生理盐水（9.0 g·L^-1）、地塞米松（1.425 mg·kg^-1）和不同剂量的扶正活血清热方（16.67，12.5，8.33 g·kg^-1）保留灌肠10次后（每日1次，5 mL/次/只），接受下腹部X射线（15 Gy）局限照射。造模24 h后麻醉处死各组大鼠，取直肠组织观察形态学改变，另取部分直肠组织液氮保存待检测组织中超氧化物歧化酶（superoxide dismustase，SOD），过氧化氢酶（catalase，CAT）活性和丙二醛（malondialdehyde，MDA）含量的变化。 结果： 在照射后1 d内模型组造模成功。组织学变化证实除模型组直肠黏膜放射后损伤病变明显，空白对照组、地塞米松组和扶正活血清热方高、中、低剂量组的直肠黏膜均无明显病变。地塞米松组和扶正活血清热方高、中、低剂量组直肠组织中SOD和CAT的酶活性水平均明显高于模型组（P<0.05），MDA含量明显低于模型组（P<0.05）。 结论： 扶正活血清热方可有效预防大鼠ARE的发生，其机制可能与阻滞放射后直肠组织中脂质过氧化反应程度有关。

Prevention Mechanism of Fuzheng Huoxue Qingre Fang on Acute Radiation Enteritis in Rats

Objective: To discuss the prevention mechanism of Fuzheng Huoxue Qingre fang(FHQF) on acute radiation enteritis(ARE) in rats. Method: Forty eight Wistar male rats were randomly divided into 6 groups(8 rats each group), blank control, model group, dexamethasone group, high,medium, and low dosage of FHQF groups. Except for the blank control, the physiological saline(9.0 g·L^-1), dexamethasone(1.425 mg·kg^-1) and FHQF(16.67,12.5,8.33 g·kg^-1) were administrated daily separately 10 days before the limit abdominal irradiation(15 Gy) by rectal clysma once a day,5 mL per rat. The morphological differences, and the levels of superoxide dismustase(SOD), catalase(CAT) and malondialdehyde(MDA) in the rectum were detected at the moment 24 hours after the modeling success of ARE in model group. Result: The modeling success of model group was observed within 1 day after the limit abdominal irradiation. No obvious morphologic differences were observed in all the groups except model group. The rectum levels of SOD and CAT in dexamethasone group, and high,medium, and low dosage of FHQF groups were found significantly (P<0.05) higher and MDA levels were significantly lower than model group(P<0.05). Conclusion: FHQF could attenuat ARE in rats effectively,whose possible prevention mechanism partly related to its powerful effects on limiting the peroxidation in the radiation injured rectum.