胎儿人细小病毒B19感染与孕妇自然流产及胎儿先天性畸形的关系

目的 探讨胎儿人细小病毒B19（HPV B19）感染与孕妇自然流产、胎儿先天性畸形的关系。方法（1）以巢式聚合酶链反应法检测182例自然流产的胚胎组织标本（自然流产组）中HPV B19DNA,以40例正常的胚胎组织标本为自然流产对照组。（2）以巢式聚合酶链反应法检测66例先天性心脏病患儿的心肌组织标本（心肌组）HPV B19 DNA,同时收集37例先天性心脏病患儿的外周血与之配对行HPV B19-IgM的酶联免疫吸附试验检测;以30例非先天性心脏病患儿的心肌标本为心肌对照组。结果 （1）自然流产组中HPV B19 DNA阳性率为25.8%（47/182）,而自然流产对照组阳性率为5.0%(2/40)。（2）心肌组中HPV B19 DNA阳性率为18.2%(12/66),而心肌对照组阳性率为0%（0/30）。（3）配对标本中心肌HPV B19 DNA阳性7例,其外周血中HPV B19-IgM全部阴性。结论 HPV B19宫内感染可能与自然流产和先天性心脏病的发生有一定关系。

Relationship between human parvovirus B19 infection and spontaneous abortion and congenital heart disease in China

Objective To explore the status of human parvovirus B19(HPV B19) infection to the fetus in China and the relationship between HPV B19 infection and spontaneous abortion,and congenital heart diseas (CHD). Methods Nest polymerase chain reaction (PCR) and in situ hybridization (ISH) were used to detect HPV B19 DNA in the tissues of spontaneous abortion and cardiac muscle of CHD, enzyme linked immunosorbent assay (ELISA) to detect HPV B19 IgM Results (1) The positive rate of HPV B19 DNA was 25 8%(47/182) in spontaneous abortion, comparing with artificial abortion 5 0%(2/40) (2) The positive rate in cardiac muscle tissues of CHD in autopsy and biopsy were 17 2%(5/29) and 18 9%(7/37), respectively The total rate was 18 2% (12/66), but the positive case was not found in control group(0/30) The HPV B19 gene were found locating in the nucleus of cardiac cell by ISH The HPV B19 IgM was negative in the blood in all patients when the HPV B19 DNA positive in the cardiac cell of biopsy Conclusion (1)There may be a relationship between HPV B19 infection and spontaneous abortion and CHD. (2) Two spontaneous abortion specimens from one person in the different period were both positive It indicated that, there was a persistent infection It may be one cause in frequently abortion