Expression of odontogenic ameloblast-associated protein, amelotin, ameloblastin, and amelogenin in odontogenic tumors: immunohistochemical analysis and pathogenetic considerations |
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Authors: | Crivelini Marcelo Macedo Felipini Renata Callestini Miyahara Glauco Issamu de Sousa Suzana Cantanhede Orsini Machado |
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Affiliation: | 1. Department of Pathology and Clinical Propaedeutic, Ara?atuba School of Dentistry, Unesp – Univ Estadual Paulista, SP, Brazil;2. Department of Stomatology, School of Dentistry, Usp – Univ Sao Paulo, SP, Brazil |
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Abstract: | Screening for expression of amelogenesis-related proteins represents a powerful molecular approach to characterize odontogenic tumors and investigate their pathogenesis. In this study, we have examined the presence and distribution of odontogenic ameloblast-associated protein (ODAM), amelotin (AMTN), ameloblastin (AMBN), and amelogenin (AMEL) by immunohistochemistry in samples of adenomatoid odontogenic tumor (AOT), calcifying epithelial odontogenic tumor (CEOT), developing odontoma, ameloblastoma, calcifying cystic odontogenic tumor (CCOT), ameloblastic fibroma (AF), myxoma, odontogenic fibroma (OF), and reduced enamel epithelia (REE). Positive results were obtained in those tumors with epithelial component, except for AF, OF, and ameloblastoma. ODAM was found around mineralized structures (dystrophic calcifications) and CEOT's amyloid, whereas AMTN stained the eosinophilic material of AOTs. The CCOT transitory cells to ghost cells were strongly positive with all proteins except AMEL, and the REE as well as odontomas showed immunoexpression for ODAM, AMTN, AMBN, and AMEL similar to those found in normal rat tooth germs. Based on these results, some histopathogenetic theories were formulated. |
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Keywords: | dental enamel proteins immunohistochemistry odontogenic ameloblast‐associated protein odontogenic tumor |
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