| CHO细胞表达重组HBsAg聚合蛋白的研究 |
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| 引用本文： | 王群,王妍,叶世德,郭铁生,陈万革,罗璇. CHO细胞表达重组HBsAg聚合蛋白的研究[J]. 中国生化药物杂志, 2000, 21(4): 180-183 |
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| 作者姓名： | 王群 王妍 叶世德 郭铁生 陈万革 罗璇 |
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| 作者单位： | 1. 卫生部长春生物制品研究所,130062
2. 吉林省中医中药研究院药厂 |
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| 摘 要： | 目的 :为提高重组乙肝疫苗的产量与质量 ,研究了纯化工艺最后一步凝胶过滤柱层析的重组HBsAg聚合蛋白。方法 :采用超声与DNA酶处理 ,凝胶过滤柱层析的步聚回收重组HBsAg。结果 :重组HBsAg纯化工艺的终收率从 35 %左右提高到 48%。重组乙肝疫苗的细胞免疫原性有了增强。结论 :以回收的重组HBsAg制备的重组乙肝疫苗在纯度、免疫原性和稳定性方面达到了世界卫生组织的标准。
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| 关 键 词： | 重组HBsAg 凝胶过滤柱层析 DNA |
| 修稿时间： | 1999-09-03 |
Study on Aggregate Protein of Recombinant HBsAg Excreted by CHO Cell |
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| Wang Qun,Wang Yan,Ye Shide,Guo Tiesheng,Chen Wange,Luo Xuan. Study on Aggregate Protein of Recombinant HBsAg Excreted by CHO Cell[J]. Chinese Journal of Biochemical Pharmaceutics, 2000, 21(4): 180-183 |
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| Authors: | Wang Qun Wang Yan Ye Shide Guo Tiesheng Chen Wange Luo Xuan |
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| Affiliation: | Changchun 130062 |
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| Abstract: | Purpose:To study the aggregate protein of recombinant HBsAg obtained from the last step of purification process,gel filtration chromatography,in order to raise the yield of recombinant hepatitis B vaccine.Methods:Ultrasonic,DNA enzyme and gel filtration chromatography were performed to recovery recombinant HBsAg.Results:The total recovery of the final r HBsAg products was raised from 35% to 48% and the cell immunity of recombinant hepatitis B vaccine was enhanced.Conclusion:The purity,immunity and stability of vaccine reached the referred criteria of WHO. |
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| Keywords: | Recombinant HBsAg Gel filtration chromatography DNA |
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