pokemon蛋白在胃上部癌组织中的表达对判断外科分子切缘的意义

目的探讨以pokemon蛋白判断胃上部癌手术切缘的安全距离。方法对60例胃上部癌，在肿瘤中心以及距上下切缘1．0、1．5、2．0、2．5、3．0、3．5、4．0、4．5及5．0cm处分别取材。采用Westernblot法检测pokemon蛋白的表达情况。结果60例胃癌组织中，pokemon蛋白表达阳性38例，表达阳性率为63．3％。与肿瘤中心处比较，上下切缘距离为1．0～2．0cm处组织中pokemon蛋白表达阳性率的差异均无统计学意义（P≥0．003），而2．5～5．0cm处组织中pokemon蛋白的表达阳性率均较低（P〈0．003）。上下切缘距离达3．5cm及以上处组织中pokemon蛋白的表达阳性率均为0。上下切缘对应位置pokemon蛋白的表达阳性率比较差异均无统计学意义（P〉0．05）。结论以pokemon蛋白的表达情况作为对胃上部癌组织切缘的研究依据，结果提示3．5cm及以外的组织pokemon蛋白的表达与正常组织无差异，可能对外科手术切缘的确定具有一定指导意义。

Expression of Pokemon Protein Predicts The Surgical Molecular Margin in Upper Gastric Cancer

Objective To investigate the safe distance of upper gastric cancer by pokemon protein. Methods In 60 cases of upper gastric cancer, specimens were get from tumors＇ tissue and every 0, 5 cm from the proximal of tumor margin beginning at the 1.0 cm to distal. Western blot method was used to test the expression ofpokemon protein. Results There were 38 cases （63.3%） positively expressed pokemon protein. Compared with the center of tumor, the positive expression rates of pokemon protein at every sites of 2.5-5.0 cm from margin were lower （P〈 O. 003）, but didn＇t signi- ficantly differed at every sites of 1.0 2.0 cm from margin （P~〉0. 003）. When the incisal margin distance of more than 3.5 cm of both sides, the positive expression rate of pokemon protein were 0. There were no significant difference between the corresponding sites of the upper resection margin and lower resection margin （P 〉 0. 05）. Conclusions The results show that 3.5 cm may be the molecular margin basing on the positive expression ofpokemon protein in the surgical margin of upper gastric cancer, which may have guiding significance to the surgical margin.