三七总皂苷对杠柳毒苷药代动力学的影响

目的]建立液相色谱-质谱联用(LC-MS/MS)定量分析方法,考察三七总皂苷对杠柳毒苷在大鼠体内的药动学影响。方法]将18只大鼠随机分成杠柳毒苷单独给药组、杠柳毒苷和低剂量三七总皂苷配伍组、杠柳毒苷和高剂量三七总皂苷配伍组,大鼠连续口服给药7 d,在最后1次给药前和给药后不同的时间点从眼底静脉丛取血,采用LC-MS/MS法,以补骨脂素为内标,测定大鼠体内杠柳毒苷的含量,并运用DAS 1.0软件计算待测物的药动学参数。结果]杠柳毒苷在1~500 ng/mL的范围内线性关系良好,方法学符合要求。药动学结果表明,杠柳毒苷配伍三七总皂苷后,杠柳毒苷在大鼠血浆中的主要药动学参数C_(max)、AUC_(0-t)、AUC_(0-∞)与杠柳毒苷单独给药相比存在统计学差异。结论]本研究所建立的LC-MS/MS方法快速、灵敏、准确,可用于杠柳毒苷在大鼠体内的药动学研究。三七总皂苷对杠柳毒苷在大鼠体内的药动学存在影响。

Effects of Panax notoginsenosides on pharmacokinetics of Periplocin

Objective] To establish a LC-MS/MS method for determination of Periplocin and study the effect of Panax notoginsenosides on pharmacokinetics of Periplocin in rats.Methods] Eighteen rats were divided into Periplocin alone group; Periplocin combined with low-dose and high-dose Panax notoginsenosides groups. All rats were orally administered for seven consecutive days, blood samples were obtained from orbitalis at different time before and after the last time administration. LC-MS/MS method was developed and validated for the determination of Periplocin in rat plasma using psoralen as the internal standard, and main pharmacokinetic parameters of analyte were processed by DAS1.0 software.Results] Calibration curves of Periplocin were linear over 1~500 ng/mL, the method validation was in line with requirements. Results of pharmacokinetics showed that there were significant differences between the pharmacokinetic parameters of Periplocin in rats with or without Panax notoginsenosides.Conclusion] The LC-MS/MS method was rapid, sensitive and accurate, and it was successfully applied to evaluate the pharmacokinetics of Periplocin in rats. And the Panax notoginsenosides had effect on pharmacokinetic of Periplocin.