| Dual Effect of 3, 4-Dihydroxyacetophenone on LPS-induced Apoptosis in RAW264.7 Cells by Modulating the Production of TNF-α |
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| 引用本文： | 吴萍,叶笃筠,张代娟,张力,万敬员,盘茜.Dual Effect of 3, 4-Dihydroxyacetophenone on LPS-induced Apoptosis in RAW264.7 Cells by Modulating the Production of TNF-α[J].华中科技大学学报(医学英德文版),2005,25(2):131-134. |
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| 作者姓名： | 吴萍 叶笃筠 张代娟 张力 万敬员 盘茜 |
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| 作者单位： | DepartmentofPathophysiology,TongjiMedicalCollege,HuazhongScienceandTechologyUniversity,Wuhan430030,China |
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| 摘 要： | To explore the pharmacological effect of 3,4-dihydroxyacetophenone (DHAP) on the apoptosis of RAW264. 7 macrophage cells and the mechanism, RAW264. 7 macrophage cells were treated with 100 or 500 mg/L lipopolysaccharide (LPS), with or without 10^-5 mol/L DHAP for 24 h. Trypan blue dye exclusion assay was used to assess cell viability. Cell apoptosis was morphological studied and flow cytometric assay was used. Tumor necrosis factor-α (TNF-α) level was measured by ELISA methods. IκB protein was determined by Western blotting. Our results showed that in 100 mg/L LPS stimulated macrophages, DHAP enhanced the cell apoptosis while in 500 mg/L LPS-stimulated macrophages, DHAP significantly inhibited the cell apoptosis. In both groups, DHAP increased the level of IκB but decreased the level of TNF-α. It is concluded that DHAP has dual effect on the apoptosis of RAW 264.7 cells treated with different concentrations of LPS. This effect may be due to the inhibition of activation of NF-κB and autocrine production of TNFα. Our study suggests that DHAP may have anti-inflammatory effect on LPS-activated macrophages.
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| 关 键 词： | 3 4-二羟基苯乙酮 药理学 RAW264 L脂多糖 巨噬细胞 |
| 收稿时间： | 13 February 2004 |
Dual effect of 3, 4-dihydroxyacetophenone on LPS-induced apoptosis in RAW264. 7 cells by modulating the production of TNF-α |
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| Wu Ping,Ye Duyun,Zhang Daijuan,Zhang Li,Wan Jingyuan,Pan Qian.Dual effect of 3, 4-dihydroxyacetophenone on LPS-induced apoptosis in RAW264. 7 cells by modulating the production of TNF-α[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2005,25(2):131-134. |
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| Authors: | Wu Ping Ye Duyun Zhang Daijuan Zhang Li Wan Jingyuan Pan Qian |
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| Institution: | (1) Present address: Department of Pathophysiology, Tongji Medical College, Huazhong Science and Technology University, 430030 Wuhan, China |
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| Abstract: | Summary To explore the pharmacological effect of 3,4-dihydroxyacetophenone (DHAP) on the apoptosis of RAW264. 7 macrophage cells and
the mechanism, RAW264. 7 macrophage cells were treated with 100 or 500 mg/L lipopolysaccharide (LPS), with or without 10−5 mol/LDHAP for 24 h. Trypan blue dye exclusion assay was used to assess cell viability. Cell apoptosis was morphological studied
and flow cytometric assay was used. Tumor necrosis factor-α (TNF-α) level was measured by ELISA methods. IϰB protein was determined
by Western blotting. Our results showed that in 100 mg/L LPS-stimulated macrophages, DHAP enhanced the cell apoptosis while
in 500 mg/L LPS-stimulated macrophages, DHAP significantly inhibited the cell apoptosis. In both groups, DHAP increased the
level of IκB but decreased the level of TNF-α. It is concluded that DHAP has dual effect on the apoptosis of RAW 264. 7 cells
treated with different concentrations of LPS. This effect may be due to the inhibition of activation of NF-κB and autocrine
production of TNFα. Our study suggests that DHAP may have anti-inflammatory effect on LPS-activated macrophages.
WU Ping, female, born in 1973, Doctoral Candidate
This project was supported by grants of the National Natural Scientific Foundation of China (No. 30070929) and (No. 30100226). |
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| Keywords: | 3 4-dihydroxyacetophenone apoptosis macrophage TNF-α NF-κ B inflammation |
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