| 联合检测端粒酶和CK20诊断大肠癌外周血微转移的研究 |
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| 引用本文： | 郭建平,孔令言. 联合检测端粒酶和CK20诊断大肠癌外周血微转移的研究[J]. 中华肿瘤防治杂志, 2005, 12(13): 979-981 |
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| 作者姓名： | 郭建平 孔令言 |
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| 作者单位： | 粤北人民医院肿瘤外科,广东,韶关,512025;粤北人民医院肿瘤外科,广东,韶关,512025 |
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| 基金项目： | 韶关市卫生科研计划项目(y04024) |
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| 摘 要： | 目的联合检测细胞角蛋白20(cytoker-atin20,CK20)表达和端粒酶的活性,初步探讨它们在检测大肠癌微转移中的意义。方法用RT-PCR法,检测52例大肠癌患者术前和术后不同时间外周血中的CK20的表达。用端粒重复片段扩增方法检测相应患者外周血中的端粒酶的活性。结果52例患者外周血中检出CK20表达及端粒酶活性检测阳性率按不同时间段分别为61·9%与57·7%(术前),69·1%与72·1%(术后24h),34·6%与30·9%(术后早期化疗结束后)。两者的阳性表达均与患者Duke’s分期存在显著性相关。结论端粒酶和CK20均可作为标志物来检测大肠癌患者外周血中微转移。
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| 关 键 词： | 结直肠肿瘤/酶学 端粒 末端转移酶/分析 肿瘤转移/血液 逆转录聚合酶链反应 |
| 文章编号： | 1009-4571(2005)13-0979-03 |
| 修稿时间： | 2005-02-21 |
Study of colorectal carcinoma micrometastases in peripheral blood by using cytokeratin 20 and telomerase activity |
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| GUO Jian-ping,KONG Ling-yan. Study of colorectal carcinoma micrometastases in peripheral blood by using cytokeratin 20 and telomerase activity[J]. Chinese Journal of Cancer Prevention and Treatment, 2005, 12(13): 979-981 |
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| Authors: | GUO Jian-ping KONG Ling-yan |
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| Abstract: | OBJECTIVE:To detect cytokeratin 20(CK20) mRNA, telomerase activity and their correlation with micrometastasis of colorectal carcinoma. METHODS: CK20 mRNA and telomerase activity in peripheral blood of 52 patients with colorectal carcinomas were detected at different time points before and after operation by RT-PCR and TRAP respectively. RESULTS: At different phases, the positive rates of CK20 mRNA and telomerase activity of those patients were 61.9% vs 57.7% (before operation), 69.1% vs 72.1% (24 h after operation) and 34.6% vs 30.9% (after the short-term chemotherapy) respectively. CONCLUSION: CK20 mRNA and telomerase activity may be potential tumor markers for detecting micrometastasis and recurrence in peripheral blood. |
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