当归注射液抗慢性脑缺血大鼠认知功能损害的实验研究

目的 观察当归注射液对慢性脑缺血后认知功能改变及氧化应激损伤和胆碱能系统的影响,探讨当归注射液改善慢性脑缺血认知功能的作用及机制. 方法 50只Wistar大鼠采用随机数字表法分为假手术组(n=16)、生理盐水组(n=18)及当归治疗组(n=16),后两组大鼠制成双侧颈动脉结扎慢性脑缺血模型(即2VO模型),缺血4周、干预4周后水迷宫检测3组大鼠认知功能,并取脑皮质测定氧化产物及胆碱酯酶含量. 结果 慢性脑缺血后大鼠的空间记忆能力受损,定位航行实验中第4、5天当归治疗组的隐匿平台逃避潜伏期较假手术组有所增加,但较生理盐水组明显缩短(分别为22.53±1.27、27.67±1.34),3组之间差异有统计学意义(P<0.05);当归治疗组在空间探索试验中穿越平台的次数较生理盐水组增多(分别为1.25±0.78、0.56±0.63),在平台区的停留时间延长(分别为21.99±4.97、12.80±2.88),3组之间差异有统计学意义(P<0.05);当归治疗组AchE含量有所下降,但与假手术组比较差异仍有统计学意义(P<0.05),同时,MDA含量增高,SOD活力、抑制羟自由基能力及总抗氧化能力降低,与假手术组比较差异没有统计学意义(P>0.05). 结论 当归注射液能改善慢性脑缺血的认知功能损害,其作用可能与抑制氧化应激反应有关.

Experimental study on effect of angelica injection to cognitive impairment after rat chronic cerebral ischemia

Objective To investigate the effect of angelica injection to vascular cognitive impairment,we observed the change of vascular cognitive function,oxidative damage and eholinergic system after chronic cerebral ischemia. Methods 50 rats were divided into sham operation (n=16), saline group(n=18) and angelica treatment group by random number table. The latter two grous chronic cerebral ischemia models were made by permanent bilateral occlusion of common carotid arteries. After 4 weeks' ischemia and 4 weeks' treatment, Spatial learning and memory abilities were evaluated by Morris water maze, the cortex were detected for oxidative production and acetylcholine esterase. Results After chronic cerebral ischemia, the spatial learning and memory abilities were destroyed, the latency of angelica treatment group in the fouth and fifth day'Morris water maze obviously shortened (27.67±1.34、22.53±1.27), and had significant difference with normal saline group(36.03±1.94、31.05±1.89). In spatial exploring testing the frequency of crossing platform of angelica treatment group increased(1.25±0.19) and the residence time prolonged(21.99±1.24). The contents of acetylcholine esterase increased after chronic cerebral ischemia (0.33±0.04)and the contents of angelica treatment group (0.29±0.05)descended, but had no significant difference. The oxidative production of normal saline group increased, the contents of MDA increased(3.37±0.22), the vigor of SOD(38.24±1.53)and the ability of inhibiting hydroxy radical (18.53±1.18) and total antioxidant (0.96±0.06)decreased. Angelica injection can inhibit oxidative production (MDA 2.38±0.18,the vigor of SOD 60.13±3.92,the ability of inhibiting hydroxy radical 30.76±2.47 ,the ability of total antioxidant 1.60±0.15),and had significant difference with normal saline group. Conclusion Angelica injection could improve cognitive function by inhibiting oxidative stress.