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| 改良Golgi-Cox染色法在大鼠脑缺血研究中的应用 | |
| 引用本文： | 李方,张新江,张苏明,方思羽. 改良Golgi-Cox染色法在大鼠脑缺血研究中的应用[J]. 西北国防医学杂志, 2002, 23(2): 85-86,F003 |
| 作者姓名： | 李方 张新江 张苏明 方思羽 |
| 作者单位： | 1. 广东省中山市南头广济医院内儿科,广东,中山市,528400 2. 兰州军区兰州总医院神经内科 3. 华中科技大学同济医学院附属同济医院神经内科 |
| 基金项目： | 国家自然科学基金重点项目 (3 973 0 170 )，面上项目(3 9770 810 )资助 |
| 摘 要： | 目的：改良Golgi-Cox染色法，使操作时间缩短、染色恒定；探讨该方法在脑缺血研究中的应用价值。方法：通过多聚甲醛固定组织、缩短浸胶时间、组织块外包石蜡壳等方法，比较研究了改良法与传统方法在显示神经元数量、操作时间等方面的差别；比较研究了大鼠脑缺血1h再灌注2、4、8和12周缺血侧神经元数量等的动态变化。结果：改良后使操作时间缩短为5－6h；染色神经元数量明显多于传统方法；脑缺知后缺血区神经元数量呈进行性减少，缺血周围残存的神经元突起增加。结论：改良Golgi-Cox神经元染色法较恒定，且省时，在脑缺血后神经元可塑性研究中有较大的应用价值。 |
| 关 键 词： | 脑缺血 神经元 高尔基染色法 大鼠 Golgi－Cox染色法 |
| 文章编号： | 1007-8622(2002)02-0085-02 |
Improvement of Golgi-Cox staining and its application in the study of cerebral ischemia of rats |
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| LI Fang,ZHANG Xin jiang,ZHANG Su ming,et al.. Improvement of Golgi-Cox staining and its application in the study of cerebral ischemia of rats[J]. Medical Journal of National Defending Forces in Northwest China, 2002, 23(2): 85-86,F003 | |
| Authors: | LI Fang ZHANG Xin jiang ZHANG Su ming et al. |
| Affiliation: | LI Fang,ZHANG Xin jiang,ZHANG Su ming,et al . |
| Abstract: | Objective:To improve the method of Golgi-Cox staining and get it more convenient in the studing of pathogenesis of ischemia in rats.Methods:In the improved group ,the rat brains were fixated by 4% paraformaldelhyde fixative in 1,2,4,12 weeks after one hour of middle cerebral artery occlusion,then immerse the brain in 5% collodion solution for 60 -120 min. A rind of paraffin was made outdise the blok.The neurons in the field of ischemia were numbered.Reults:After improving,the procedure was decreased to 5-6 hours.Moreover, using this method , the number of stained neurons was stable in the rat brain.It was decreased gradually after ischemia,but the damaged neuron had more dendrites in the perilesion.Conclusion:The method is not only saving time,but also more stable than the one reported before.It may be used in the studing of plasticity after ischemia. |
| Keywords: | Cerebral ischemia Neuron Golgi-Cox staining Rat |
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