| 穿山龙提取物的降血尿酸作用及醇提工艺研究 |
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| 引用本文： | 周忠东,王建平,钱莺,傅旭春,白海波,扈荣,刘二刚.穿山龙提取物的降血尿酸作用及醇提工艺研究[J].中国现代应用药学,2014,31(6):699-702. |
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| 作者姓名： | 周忠东 王建平 钱莺 傅旭春 白海波 扈荣 刘二刚 |
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| 作者单位： | 绍兴市中医院，浙江 绍兴 312000;浙江省中医院，杭州 310006;浙江大学医学院附属妇产科医院，杭州 310006;浙江大学城市学院药物研究所，杭州 310015;杭州华东医药集团生物工程研究所有限公司，杭州 310012;杭州华东医药集团生物工程研究所有限公司，杭州 310012;中国科学院上海药物研究所，上海 201203；天津大学化工学院化学工程联合国家重点实验室，天津 300072 |
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| 基金项目： | 杭州市科技发展计划项目(20100331T01) |
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| 摘 要： | 目的 研究穿山龙降血尿酸有效成分的提取工艺。方法 应用大鼠急性高尿酸血症模型评价穿山龙提取物的降血尿酸作用。以穿山龙中所含的薯蓣皂苷为指标，用正交试验法考察乙醇浓度、提取时间、提取次数和溶剂用量4种因素对提取结果的影响。薯蓣皂苷和血尿酸的含量均用HPLC测定。结果 穿山龙乙醇提取物具有降血尿酸作用。只有提取次数对穿山龙的薯蓣皂苷提取率有显著影响。结论 穿山龙降血尿酸有效成分的最佳提取工艺为：6倍量50％乙醇提取3次，每次1 h。
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| 关 键 词： | 穿山龙 薯蓣皂苷 血尿酸 提取工艺 正交设计 |
| 收稿时间： | 2013/6/19 0:00:00 |
| 修稿时间： | 2014/1/22 0:00:00 |
Study on the Hypouricemic Effect of Extracts from Dioscorea Nipponicae Rhizoma and Extracting Process of Its Ethanol Extract |
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| ZHOU Zhongdong,WANG Jianping,QIAN Ying,FU Xuchun,BAI Haibo,HU Rong and LIU Ergang.Study on the Hypouricemic Effect of Extracts from Dioscorea Nipponicae Rhizoma and Extracting Process of Its Ethanol Extract[J].The Chinese Journal of Modern Applied Pharmacy,2014,31(6):699-702. |
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| Authors: | ZHOU Zhongdong WANG Jianping QIAN Ying FU Xuchun BAI Haibo HU Rong and LIU Ergang |
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| Institution: | Shaoxing Traditional Chinese Medical Hospital, Shaoxing 312000, China;Zhejiang Traditional Chinese Medical Hospital, Hangzhou 310006, China;Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 31006, China;Institute of Materia Medical, Zhejiang University City Collegy, Hangzhou 310015, China;Hangzhou Huadong Medicine Group Biotechnology R&D Institute Co.,Ltd., Hangzhou 310012, China;Hangzhou Huadong Medicine Group Biotechnology R&D Institute Co.,Ltd., Hangzhou 310012, China;Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China |
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| Abstract: | OBJECTIVE To optimize the extracting process of ethanol extracts with hypouricemic effect from Dioscorea Nipponicae Rhizoma. METHODS The hyperuricemia model of rats was utilized to evaluate the hypouricemic effect of ethanol extracts from Dioscorea Nipponicae Rhizoma. Orthogonal design was utilized to optimize the preparing conditions including the concentration of ethanol, amount of ethanol, extraction times and extraction duration per time when dioscin was selected as the evaluation index. Both dioscin and serum uric acid levels were measured by HPLC. RESULTS The ethanol extracts of Dioscorea Nipponicae Rhizoma had hypouricemic action. Only extraction times had significant effect on the content of dioscin. CONCLUSION The optimum extraction process was to extract three times using six-fold 50% ethanol and 1 h per time. |
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| Keywords: | Dioscorea Nipponicae Rhizoma dioscin serum uric acid extraction process orthogonal design |
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