| 江苏人群19个STR基因座的遗传多态性 |
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| 引用本文： | 居晓斌,潘 猛,刘燕婷,周 蓉,陈 奇,霍 然,周惠英.江苏人群19个STR基因座的遗传多态性[J].南京医科大学学报,2014(6):750-754-792. |
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| 作者姓名： | 居晓斌 潘 猛 刘燕婷 周 蓉 陈 奇 霍 然 周惠英 |
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| 作者单位： | 南京医科大学第一附属医院司法鉴定所,江苏 南京 210029,南京医科大学基础医学院人体解剖与组织胚胎学系,江苏 南京 210029;南京医科大学第一附属医院司法鉴定所,江苏 南京 210029;南京医科大学第一附属医院司法鉴定所,江苏 南京 210029;南京医科大学第一附属医院司法鉴定所,江苏 南京 210029;南京医科大学第一附属医院司法鉴定所,江苏 南京 210029;南京医科大学基础医学院人体解剖与组织胚胎学系,江苏 南京 210029;南京医科大学第一附属医院司法鉴定所,江苏 南京 210029 |
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| 基金项目： | 国家自然科学基金(31171113);国家973计划项目(2006CB504002) |
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| 摘 要： | 目的:利用3130遗传基因测序仪的电泳图谱分析江苏人群D21S11?D6S1043?Penta E?PGA?D2S1338?D11S51?D19S433?D12S391?CSF1PO?D3S1358?Penta D?VWA?D13S317?THO1?TPOX?D8S1179?D16S539?D5S818?D7S820 19个STR基因座多态性分布,并计算该19个基因座的基因频率?个体鉴别能力?无偏倚期望杂合度?多态性信息总量和非父排除率?方法:利用 PCR扩增,产物通过3130测序仪毛细管电泳?读出数据后用分析软件进行分析?结果:19个STR位点中Penta E的个体鉴别能力值和非父排除率值最高,19个STR位点的累积非父排除率达0.9999以上?结论:通过大量的样本实验和等位基因频率的统计,得出了更加客观的等位基因多态性数据,为江苏地区亲权鉴定的判定提供更加客观的依据?
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| 关 键 词： | 短串联重复序列 亲权鉴定 遗传多态性 |
| 收稿时间： | 2014/1/10 0:00:00 |
The polymorphism distributions of nineteen STR loci in Jiangsu population |
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| Ju Xiaobin,Pan Meng,Liu Yanting,Zhou Rong,Chen Qi,Huo Ran and Zhou Huiying.The polymorphism distributions of nineteen STR loci in Jiangsu population[J].Acta Universitatis Medicinalis Nanjing,2014(6):750-754-792. |
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| Authors: | Ju Xiaobin Pan Meng Liu Yanting Zhou Rong Chen Qi Huo Ran and Zhou Huiying |
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| Institution: | The Institute of Judicial Expertise,the First Affiliated Hospital of NJMU,Nanjing 210029,Basic Medical Sciences of Anatomy and Embryology,NJMU,Nanjing 210029,China;The Institute of Judicial Expertise,the First Affiliated Hospital of NJMU,Nanjing 210029;The Institute of Judicial Expertise,the First Affiliated Hospital of NJMU,Nanjing 210029;The Institute of Judicial Expertise,the First Affiliated Hospital of NJMU,Nanjing 210029;The Institute of Judicial Expertise,the First Affiliated Hospital of NJMU,Nanjing 210029;Basic Medical Sciences of Anatomy and Embryology,NJMU,Nanjing 210029,China;The Institute of Judicial Expertise,the First Affiliated Hospital of NJMU,Nanjing 210029 |
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| Abstract: | Objective:To analyze the polymorphism distributions of 19 STR loci including D21S11,D6S1043,Penta E,PGA,D2S1338,D11S51,D19S433,D12S391,CSF1PO,D3S1358,Penta D,VWA,D13S317,THO1,TPOX,D8S1179,D16S539,D5S818 and D7S820 in Jiangsu population,and calculate their gene frequency(P),power of discrimination(DP),unbiased expected heterozygosity(H),polymorphism information content(PIC),and probability of paternity exclusion(PE). Methods:Capillary electrophoresis was detected using PCR via 3130 sequencing. Analysis software was used to analyze data. Results:The DP value and PE value of Penta E were the highest in the 19 STR sites. The cumulate PE of the 19 STR loci was above 0.9999. Conclusion:Objective allelic polymorphism data were obtained from a large number of sample experiments and allele frequency,which provide an objective basis for identification in disputed paternity of Jiangsu area. |
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| Keywords: | short tandem partentage testing genetic polymorphism |
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