菟丝子多糖对衰老小鼠脂质过氧化的影响

目的:观察菟丝子多糖对衰老小鼠模型丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSHPX)和脑组织中脂褐质(LF)的影响。方法:用d半乳糖制备衰老小鼠模型,随机分为模型组(不给药)、阳性对照组(灌注维生素E200mg·kg-1,qd)、3个剂量菟丝子多糖组(分别灌注100,200,400mg·kg-1菟丝子多糖,qd),共7wk。另设空白对照组。结果:模型小鼠血清、肝、肾中MDA含量升高,SOD及GSHPX活性降低,脑中LF升高。而100,200,400mg·kg-1·d-1菟丝子多糖组能不同程度对抗模型小鼠脑组织中LF升高及血清、肝、肾组织中MDA升高,其量效关系均呈负相关,r分别为-0.916,-0.939,-0.950及-0.911,P<0.01;能不同程度抑制血清、肝、肾组织中SOD活力下降,其量效关系均呈正相关,r分别为0.898,0.939及0.946,P<0.01;能不同程度抑制血清、肝、肾组织中GSHPX活力下降,其量效关系均呈正相关,γ分别为0.947,0.969及0.947,P<0.01。结论:菟丝子多糖有显著清除氧自由基及抗脂质过氧化作用。

Effect of polysaccharide from Cuscuta chinensis on lipoperoxidation of senile mice

To observe the effects of polysaccharide from Cuscuta chine nsis (PCC)on malondialdehyde (MDA), superoxide dismutase (SOD) and glutat hi one peroxidase (GSH-PX) in serum, liver, kidney and lipofuscin(LF) in brain of senile model mice. METHODS: The senile model mice caused by d -galatose (d-gal), were randomly divided into model group (no administer d rug), positive control group (gavage Vit E 200 mg·kg~(-1),qd), three differe nt doses of PCC groups (gavage PCC 100,200,400 mg·kg~(-1),qd, respectively ) for 7 wk. Another blank group was controled. RESULTS:The model group showed elevations of MDA in serum, liver, kidney and LF in brain; conversely with descent of SOD and GSH-PX activities. The three diff erent doses of PCC groups demonstrated different degrees of antagonistic negativ e correlation ship for dosage effects on elevations of cerebral LF, and the MDA in serum, liver, kidney of model mice, with -0.916,(-0.939),(-0.950) and (-0.911) r, respec tively, P<0.01; furthermore revealid various inhibitions on descend activiti es of SOD and GSH-PX in serum, liver, kidney with dosage effects of positive co rrelationship 0.898, 0.939 and(0.946) r,P<(0.01); and (0.947),( 0.969), (0.947)[JP] r,(P<0.01), respectively. CONCLUSION: PCC possesses the notable effects of eliminating oxyen free radicals and antilipoperoxidation.